Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24537||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4731
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt nameCaspase-3 FRET reporter
Alt nameeffector caspase reporter protein (EC-RP)
Insert Size (bp)792
- Promoter CMV
/ Fusion Proteins
- Cloning method Restriction Enzyme
- 5′ cloning site BspEI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
Effector caspase reporter protein (EC-RP) used to monitor caspase-3 activity (and caspase-7 activity to a lesser extent). EC-RP construct is composed of a FRET donor-acceptor pair ECFP and Venus connected via a flexible linker (SGLRSSGDEVDRVYGSGS) that contains the caspase cleavage sequence DEVDR. When the linker is cleaved, energy transfer is lost and the ECFP signal increases. The DEVDR linker in EC-RP is expected to have 20-fold greater selectivity for caspase-3 relative to caspase-8 than the DEVDG linker used in other caspase reporters.
EC-RP was constructed by ligating Venus (YFP) between BamHI and EcoRI sites in pECFP-C1 and ligating linkers encoding cleavage sequences as BspEI-BamHI fragments between ECFP and Venus. Multiple serine and glycine residues flanking the cleavage sequences were added to increase linker flexibility and substrate accessibility. See https://www.ncbi.nlm.nih.gov/pubmed/18406323 for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pECFP-DEVDR-Venus was a gift from Peter Sorger (Addgene plasmid # 24537 ; http://n2t.net/addgene:24537 ; RRID:Addgene_24537)
For your References section:Modeling a snap-action, variable-delay switch controlling extrinsic cell death. Albeck JG, Burke JM, Spencer SL, Lauffenburger DA, Sorger PK. PLoS Biol. 2008 Dec 2. 6(12):2831-52. 10.1371/journal.pbio.0060299 PubMed 19053173