166,461 results

1. PB-U6

   Plasmid

   #120358

   Purpose

   piggyBac transposon vector for sgRNA expression.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. NanoLuc-PM

   Plasmid

   #164784

   Purpose

   Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

   Depositor

   Laszlo Hunyady

   Article

   Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

   Insert

   Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Oplophorus gracilirostris, Human)
   

   Use

   Tags

   NanoLuc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-syn-iGluSnFR4f-PDGFR-WPRE

   Plasmid

   #234436

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. mcherry-PH

   Plasmid

   #36075

   Depositor

   Narasimhan Gautam

   Article

   Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

   Insert

   PH domain of PLCdelta1 (PLCD1 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmTurquoise2-Mito

   Plasmid

   #36208

   Purpose

   In vivo visualization of the mitochondria (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   MTS (COX8A Human)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. LeGO-V2

   Plasmid

   #27340

   Depositor

   Boris Fehse

   Article

   Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

   Insert

   SFFV promoter, Venus fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tol2-BetaActin-SecA5-YFP

   Plasmid

   #105664

   Purpose

   Ubiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporter

   Depositor

   Qing Deng

   Article

   Hsu et al Front Immunol. 2018 Mar 29;9:607. doi: 1

   Insert

   Secreted Annexin5-EYFP (ANXA5 Human)
   

   Use

   Tol2-gateway

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Beta-Actin

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. MCP-APEX2

   Plasmid

   #154936

   Purpose

   NLS-V5-MCP-APEX2

   Depositor

   Alice Ting

   Article

   Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

   Insert

   NLS-V5-MCP-APEX2
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmiRFP713-N1

   Plasmid

   #136559

   Purpose

   A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP713

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   miRFP713
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEIGW Grx1-roGFP2

    Plasmid

    #64990

    Purpose

    Mammalian expression of cytosolic Grx1-roGFP2 (lentiviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBHSTC104

    Plasmid

    #124356

    Purpose

    Plasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin

    Depositor

    Hideo Iwai

    Article

    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

    Insert

    gp41-1 N-intein and C-intein (bicistronic)
    

    Use

    Tags

    CcdA-C (SFAD)

    Expression

    Bacterial

    Mutation

    Promoter

    STC

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST14-Cys-DogCatcher

    Plasmid

    #171929

    Purpose

    Express Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    Cys-DogCatcher
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PRS_8-ChrimsonR-tdTomato

    Plasmid

    #192587

    Purpose

    AAV-vector for optogenetic activation of noradrenergic neurons

    Depositor

    Simon Wiegert

    Article

    Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    PRSX8

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1GFP/RNase H1 WT

    Plasmid

    #174447

    Purpose

    Express GFP-tagged RNase H1 WT in E. coli.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    RNase H1 (RNASEH1 Human)
    

    Use

    Tags

    His-tag, GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-HA-UTX

    Plasmid

    #24168

    Purpose

    Mammalian expression of human UTX with HA tag

    Depositor

    Kristian Helin

    Article

    Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

    Insert

    UTX (KDM6A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-2xFYVE

    Plasmid

    #140047

    Purpose

    Expresses GFP-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available Since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRRLsinPGK_IRFP720_WPRE

    Plasmid

    #91785

    Purpose

    Constitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.

    Depositor

    Beverly Torok-Storb

    Insert

    iRFP720
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCrl

    Plasmid

    #122443

    Purpose

    Expresses entire mouse reelin ORF in mammalian cells

    Depositor

    Tom Curran

    Article

    D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

    Insert

    reelin (Reln Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CLYBL-TO-hNGN2-BSD-mApple

    Plasmid

    #124229

    Purpose

    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

    Depositor

    Michael Ward

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    NGN2 (NEUROG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-Sac-Abeta(M1-42)

    Plasmid

    #71875

    Purpose

    Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

    Depositor

    Dominic Walsh

    Article

    Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

    Insert

    Abeta(M1-42) (APP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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