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Plasmid#102598PurposeExpress STING for IFN-beta luciferase reporter assay.DepositorInsertSTING (STING1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-hM4D(Gi)-mCherry
Plasmid#50479PurposeGi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoterDepositorHas ServiceAAV5InserthM4D(Gi)-mCherry
UseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9
Plasmid Kit#1000000171PurposeVectors and DNA modules for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency intron-optimized SpCas9-coding gene.DepositorAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRE-BI-hGRAD
Plasmid#207837PurposeInducible hGRAD Plasmids for rapid depletion of GFP-tagged proteins in mammalian cellsDepositorInsertsUseTagsExpressionMammalianMutation215 amino acids of the full length proteinPromoterTRE3G BI promoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-ShadowR
Plasmid#132668PurposeExpresses ShadowR in mammalian cellsDepositorInsertShadowR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVdual-CMV-eGFP
Plasmid#230931PurposepAAVdual plasmid is used to package AAV-CMV-eGFP viruses with the AAVdual system, which integrates the mini-pHelper-1.0 (providing E2A, E4orf6, and VA RNA) with an AAV expression cassette containing eGFP driven by the CMV promoter.DepositorInserteGFP
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHN924
Plasmid#178885PurposeExpression of GST-His8-HRV3CDepositorInserthuman rhinovirus 3C protease
UseTagsGST, His8ExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSFVCs-lacZ
Plasmid#92076PurposeThis is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.DepositorInsertC-lacZ
UseC-lacz expression plasmid, for run-off transcript…TagsExpressionMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_BFP
Plasmid#214679PurposeLentiviral expression vector for an inducible Cas9-P2A-BFP with two empty sgRNA cloning sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTag
Plasmid#209657PurposeExpression of iATPSnFR2, low affinityDepositorInsertiATPSnFR2.A95A.A119L.HaloTag
UseAAVTagsExpressionMutationA95A.A119LPromoterCAGAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
alpha7nACHR_Nacho_GCAMP7s
Plasmid#182940Purposealpha7 nACHR expression in mammalian cell lines with calcium reporterDepositorInsertNACH7 (CHRNA7 Human)
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2attNG-H11-short
Plasmid#51546Purposep2attNG landing pad plasmid with 400 bp homology arms to the human H11 locus (for use with TALEN-assisted recombination).DepositorInsertGFP4m
UseHuman targetingTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVoPo-GFP
Plasmid#187840PurposeConstitutive GFP-expression vector for Fusobacterium nucleatumDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0046-EF1a-PspCas13b-NES-HIV
Plasmid#103862PurposeExpresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasmDepositorInsertPspCas13b
UseCRISPR and LentiviralTags3xHA and HIV NESExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
MIGR1
Plasmid#27490DepositorTypeEmpty backboneUseRetroviralTagsGFP and IRESExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSynapsin_psychLight2
Plasmid#163909PurposeTo generate Adeno-Associated viruses for expression of psychLight2 under synapsin promoterDepositorHas ServiceAAV9InsertpsychLight2
UseAAVTagsFlag tagExpressionMammalianMutationPromoterSynapsinAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb
Plasmid#175544PurposeExpression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag9-myc_PDGFRtmb
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a-CysRS-His
Plasmid#124107PurposeBacterial expression plasmid for PURE system componentsDepositorInsertCysteinyl-tRNA ligase (cysS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)
Plasmid#79620PurposeExpresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cellsDepositorInsertSpCas9
UseTagsPmCDA1 and UGIExpressionMammalianMutationD10A for nickase Cas9PromoterpCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a-His-IleRS
Plasmid#124112PurposeBacterial expression plasmid for PURE system componentsDepositorInsertIsoleucyl-tRNA ligase (ileS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only