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Proximity labeling of protein complexes and cell type-specific organellar proteomes in Arabidopsis enabled by TurboID.
Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC
Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
127349pENTR_L1-YFP-Turbo-NES-L2gateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins
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127350pENTR_L1-YFP-Turbo-L2gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins
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127351pENTR_L1-YFP-miniTurbo-NES-L2gateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteins
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127352pENTR_L1-YFP-miniTurbo-L2gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins
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127353pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins
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127354pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins
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127355pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins
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127356pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins
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127357pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice
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127358pENTR_L1-Turbo-NES-YFP-STOP-L2gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice
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127359pENTR_L1-miniTurbo-NES-YFP-STOP-L2gateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choice
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127360pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice
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127361pENTR_L1-Turbo-YFP-NLS-STOP-L2gateway entry vector for expressing nuclear TurboID-YFP under a promoter of choice
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127362pENTR_L1-miniTurbo-YFP-NLS-STOP-L2gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice
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127363R4pGWB601_UBQ10p-BirA(R118G)-NES-YFPBinary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plants
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127365R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLSBinary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants
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127366R4pGWB601_UBQ10p-Turbo-NES-YFPBinary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plants
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127368R4pGWB601_UBQ10p-Turbo-YFP-NLSBinary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants
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127369R4pGWB601_UBQ10p-miniTurbo-NES-YFPBinary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants
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127370R4pGWB601_UBQ10p-miniTurbo-YFP-NLSBinary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants
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Recombinant Antibodies from Article