pENTR_L1-miniTurbo-NES-YFP-STOP-L2
(Plasmid
#127359)
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Purposegateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choice
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Depositing Lab
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Depositing OrganizationStanford University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127359 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 127359-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 127359-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepENTR
- Total vector size (bp) 4181
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Vector typeGateway Cloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameminiTurbo (BirA mutant)
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SpeciesBirA from E. coli, codon optimized for human and mutated
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Insert Size (bp)771
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Mutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, I305V
- Promoter no promoter
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Tags
/ Fusion Proteins
- V5 (N terminal on insert)
- NES (C terminal on insert)
- GS linker (C terminal on insert)
- YFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 fw
- 3′ sequencing primer M13 rv
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byminiTurbo was obtained from Alice Ting's lab at Stanford University (cloned from V5-miniTurbo-NES_pCDNA3, Addgene catalog #107170).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 127359-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 127359-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR_L1-miniTurbo-NES-YFP-STOP-L2 was a gift from Dominique Bergmann (Addgene plasmid # 127359 ; http://n2t.net/addgene:127359 ; RRID:Addgene_127359) -
For your References section:
Proximity labeling of protein complexes and cell type-specific organellar proteomes in Arabidopsis enabled by TurboID. Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC. Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864. 10.7554/eLife.47864 PubMed 31535972
Map uploaded by the depositor.
