Purposegateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||127349||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Total vector size (bp) 4397
Vector typegateway entry vector
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameTurboID (BirA mutant)
SpeciesBirA from E. coli, codon optimized for human and mutated
Insert Size (bp)957
MutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, M241T, S263P, I305V
- Promoter no promoter
/ Fusion Proteins
- YFP (N terminal on insert)
- GS linker (N terminal on insert)
- V5 (N terminal on insert)
- NES (C terminal on insert)
- GS linker (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer M13 fw
- 3′ sequencing primer M13 rv (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byTurboID was obtained from Alice Ting's lab at Stanford University (cloned from V5-hBirA-Turbo-NES_pCDNA3, Addgene catalog #107169).
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pENTR_L1-YFP-Turbo-NES-L2 was a gift from Dominique Bergmann (Addgene plasmid # 127349 ; http://n2t.net/addgene:127349 ; RRID:Addgene_127349)
For your References section:Proximity labeling of protein complexes and cell type-specific organellar proteomes in Arabidopsis enabled by TurboID. Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC. Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864. 10.7554/eLife.47864 PubMed 31535972