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Using CRISPR-Cas9-mediated genome editing to generate C. difficile mutants defective in selenoproteins synthesis.
McAllister KN, Bouillaut L, Kahn JN, Self WT, Sorg JA
Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.1038/s41598-017-15236-5.
PubMed

Plasmids from Article

ID Plasmid Purpose  
105133pJK02Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT
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105134pKM126Clostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriT
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