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SnoopLigase Catalyzes Peptide-Peptide Locking and Enables Solid-Phase Conjugate Isolation.
Buldun CM, Jean JX, Bedford MR, Howarth M
J Am Chem Soc. 2018 Feb 28;140(8):3008-3018. doi: 10.1021/jacs.7b13237. Epub 2018 Feb 14.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
105626pET28a-AviTag-SnoopLigasePlasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation
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105627pET28a-HaloTag7-SnoopLigasePlasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7
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105628pET28a-SnoopTagJr-MBPPlasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigase
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105629pET28a-SUMO-DogTagPlasmid for recombinant expression in bacteria of SUMO domain fused to DogTag, permitting covalent linkage to SnoopTagJr
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