|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11685||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerWahl lab
- Backbone size w/o insert (bp) 7117
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI/BglII (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer See map (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byLucGFP fragment was cloned from pLuciferase-EGFP vector (gift of D. Buscher).
Terms and Licenses
- Not Available to Industry
Article Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pL3-TRE-LucGFP-2L was a gift from Geoff Wahl (Addgene plasmid # 11685 ; http://n2t.net/addgene:11685 ; RRID:Addgene_11685)
For your References section:Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange. Wong ET, Kolman JL, Li YC, Mesner LD, Hillen W, Berens C, Wahl GM. Nucleic Acids Res. 2005 . 33(17):e147. 10.1093/nar/gni145 PubMed 16204450