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(Plasmid #163148)


Item Catalog # Description Quantity Price (USD)
Plasmid 163148 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 8635
  • Modifications to backbone
    RSF origin and kanamycin resistance were taken from pRSF-Duet1 and used to replace the pBR322 origin and ampicillin resistance of pBAD-myc-HisA
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    allo-tRNA UTu2D
  • Species
  • Insert Size (bp)
  • GenBank ID
  • Promoter araBAD

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
  • Alt name
  • Species
    Treponema denticola
  • Insert Size (bp)
  • Mutation
    Cysteine 32 switched to Selenocysteine (UAG)
  • GenBank ID

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    Selenophosphate synthase
  • Alt name
  • Species
    Aeromonas salmonicida
  • Insert Size (bp)
  • Mutation
    starts with GUG instead of AUG
  • GenBank ID
  • Promoter native As SelD promoter

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTACCACGGTGCCCAAAAGTC
  • 3′ sequencing primer GCATTTTCGACAGGCTATTG
  • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    Selenocysteine synthase
  • Alt name
  • Species
    Aeromonas salmonicida
  • Insert Size (bp)
  • Mutation
    changed initiator AUG to GUG then the following amino acids P2T, L69F, Q72S, and C173V
  • GenBank ID
  • Promoter EM7

Cloning Information for Gene/Insert 4

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAGGGCATTATGCCACAAG
  • 3′ sequencing primer GAAGTGGATCGTCTGGTGAC
  • (Common Sequencing Primers)

Gene/Insert 5

  • Gene/Insert name
    Cysteine desulfurase
  • Alt name
  • Species
    Escherichia coli
  • Insert Size (bp)
  • Mutation
    Cysteine 364 to Alanine
  • GenBank ID
  • Promoter native Ec SufS promoter

Cloning Information for Gene/Insert 5

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GATTCGGGCTGACTGTGAGAG
  • 3′ sequencing primer CTCGGTGAGTTTTCTCCTTC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For additional information and the protocol for using this plasmid, please see:

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSecUAG-Evol2 was a gift from Dieter Söll (Addgene plasmid # 163148 ; ; RRID:Addgene_163148)
  • For your References section:

    A Facile Method for Producing Selenocysteine-Containing Proteins. Mukai T, Sevostyanova A, Suzuki T, Fu X, Soll D. Angew Chem Int Ed Engl. 2018 Jun 11;57(24):7215-7219. doi: 10.1002/anie.201713215. Epub 2018 May 9. 10.1002/anie.201713215 PubMed 29631320