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pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)
(Plasmid #103849)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 103849 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 9846
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    PspCas13b
  • Species
    Prevotella sp. P5-125
  • Insert Size (bp)
    3270
  • Mutation
    Changed histidne 133 to alanine and histidine 1058 to alanine to remove RNAse activity.
  • GenBank ID
    WP_044065294
  • Promoter CMV
  • Tag / Fusion Protein
    • HIV NES (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    huADAR2DD
  • Alt name
    human ADAR2 deaminase domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1158
  • Mutation
    Only the deaminase domain (AA 276-702 are expressed). Glutamate 488 is mutated to glutamine.
  • Entrez Gene
    ADARB1 (a.k.a. ADAR2, DRABA2, DRADA2, RED1)
  • Promoter CMV
  • Tag / Fusion Protein
    • GS (N terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q) was a gift from Feng Zhang (Addgene plasmid # 103849)
  • For your References section:

    RNA editing with CRISPR-Cas13. Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180. 10.1126/science.aaq0180 PubMed 29070703