pcDNA3 MEKK1 KD
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12180||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5600
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)5200
MutationKinase dead: K1253M; V1467L
Entrez GeneMap3k1 (a.k.a. MAPKKK1, MEKK1, Mekk)
/ Fusion Proteins
- His (N terminal on insert)
- Xpress (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that Addgene's sequencing results identified a V1467L mutation in the MEKK1 sequence when compared to GenBank ID AF117340.1. The depositing laboratory states that this plasmid functions exactly as intended and the mutation may represent a valid polymorphism.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 MEKK1 KD was a gift from Gary Johnson (Addgene plasmid # 12180 ; http://n2t.net/addgene:12180 ; RRID:Addgene_12180)
For your References section:MEK kinase 1, a substrate for DEVD-directed caspases, is involved in genotoxin-induced apoptosis. Widmann C, Gerwins P, Johnson NL, Jarpe MB, Johnson GL. Mol Cell Biol. 1998 Apr . 18(4):2416-29. PubMed 9528810