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pCMV5 MEKK4alpha KD
(Plasmid #12188)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12188 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MEKK4
  • Alt name
    MEKK4 alpha
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4900
  • Mutation
    Kinase dead: K1361M
  • GenBank ID
    U85607
  • Entrez Gene
    Map3k4 (a.k.a. D17Rp17, D17Rp17e, MAPKKK4, MEKK 4, MTK1, Mek4b, Mekk4, RP17, Rp17a, Tas, mKIAA0213)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site ApaI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5 MEKK4alpha KD was a gift from Gary Johnson (Addgene plasmid # 12188 ; http://n2t.net/addgene:12188 ; RRID:Addgene_12188)
  • For your References section:

    Cloning of a novel mitogen-activated protein kinase kinase kinase, MEKK4, that selectively regulates the c-Jun amino terminal kinase pathway. Gerwins P, Blank JL, Johnson GL. J Biol Chem. 1997 Mar 28. 272(13):8288-95. 10.1074/jbc.272.13.8288 PubMed 9079650