pCMV5 MEKK4alpha WT
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12187||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4600
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameMEKK4 alpha
SpeciesM. musculus (mouse)
Insert Size (bp)4900
Entrez GeneMap3k4 (a.k.a. D17Rp17, D17Rp17e, MAPKKK4, MEKK 4, MTK1, Mek4b, Mekk4, RP17, Rp17a, Tas, mKIAA0213)
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site ApaI (destroyed during cloning)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV5 MEKK4alpha WT was a gift from Gary Johnson (Addgene plasmid # 12187 ; http://n2t.net/addgene:12187 ; RRID:Addgene_12187)
For your References section:Cloning of a novel mitogen-activated protein kinase kinase kinase, MEKK4, that selectively regulates the c-Jun amino terminal kinase pathway. Gerwins P, Blank JL, Johnson GL. J Biol Chem. 1997 Mar 28. 272(13):8288-95. 10.1074/jbc.272.13.8288 PubMed 9079650