pCMV5 MEKK2 WT
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12182||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4600
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1900
Entrez GeneMap3k2 (a.k.a. 9630061B06Rik, AI585793, Mekk2, Mekk2b)
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI? (not destroyed)
- 3′ cloning site BglII? (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV5 MEKK2 WT was a gift from Gary Johnson (Addgene plasmid # 12182 ; http://n2t.net/addgene:12182 ; RRID:Addgene_12182)
For your References section:Molecular cloning of mitogen-activated protein/ERK kinase kinases (MEKK) 2 and 3. Regulation of sequential phosphorylation pathways involving mitogen-activated protein kinase and c-Jun kinase. Blank JL, Gerwins P, Elliott EM, Sather S, Johnson GL. J Biol Chem. 1996 Mar 8. 271(10):5361-8. 10.1074/jbc.271.10.5361 PubMed 8621389