179,692 results
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Bacterial strain#182778PurposeRecoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivatedDepositorBacterial resistanceSpectinomycinAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only
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PB-TO-oNGN2
Plasmid#198397PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.InsertsTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationS24A, S193A, S207A, S209A, S219A, S232A, S239A, S…PromoterCAG, EF1a, and TRE3GAvailable sinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBITE-CD19-CD3
Plasmid#190677PurposeEncodes a modularized form of HD37-OKT3 bispecific antibodyDepositorInsertModularized-HD37-modularized-OKT3-P2A-NeonGreen
UseLentiviralTagsmNeonGreenExpressionMammalianPromoterEFSAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi 41BBL-CD86 mIL-15
Plasmid#183245PurposeExpression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cellsDepositorInsert41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)
ExpressionMammalianAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRX-No-HaloTag7-mGFP-LC3
Plasmid#184901PurposeStable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic fluxDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLC3B
TagsHaloTag7 and monomeric EGFPExpressionMammalianAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTXB1-nbMmIgG1-Tn5
Plasmid#184287PurposeAnti-mouse IgG1 Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmIgG1-Tn5
Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde)
Viral prep#162376-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP8f-WPRE (#162376). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8f-WPRE plasmid DNA. Syn-driven expression of ultrafast calcium sensor GCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-RiboL1-jGCaMP8s
Plasmid#169247PurposeAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.DepositorInsertRiboL1-jGCaMP8s
UseAAVTags6xHisExpressionMammalianPromoterhSynAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
2X_pX458_pSpCas9(BB)-2A-GFP
Plasmid#172221PurposeCas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.DepositorTypeEmpty backboneUseCRISPRTags3XFLAG and GFPExpressionMammalianPromoterCbhAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
spNW50
Plasmid#173486PurposeExpresses spNW50 in Bl21DepositorInsertspNW50
TagsHistagExpressionBacterialAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-CMV-Puro-AAVR-FLAG
Plasmid#166716PurposeVector expressing AAVR (KIAA0319L) with a C-terminal Flag tagDepositorAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSecUAG-Evol2
Plasmid#163148PurposeExpresses machinery necessary for selenocysteine incorporation at UAG codonsDepositorInsertsallo-tRNA UTu2D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
Cysteine desulfurase
ExpressionBacterialMutationCysteine 32 switched to Selenocysteine (UAG), Cys…PromoterEM7, araBAD, native As SelD promoter, and native …Available sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-Cre-P2A-dTomato (AAV2)
Viral prep#107738-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsdTomato (physically separate, not a fusion protein)Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_sgRNA_puro-mKate-lox2272
Plasmid#162076Purpose3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistanceDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralExpressionMammalianPromoterU6, EF1aAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO.1 Puro shRNA Scramble
Plasmid#162011PurposeLentiviral negative control vector containing scramble shRNADepositorInsertscramble shRNA
UseLentiviralAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA-FLAG-METTL3
Plasmid#160250PurposeExpress FLAG-METTL3 in human cellsDepositorAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV PHP.eB)
Viral prep#20298-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Cre-dependent)Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Dcp1a
Plasmid#153972PurposeFluorescent protein fused to mRNA decapping enzyme 1aDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmRNA decapping enzyme 1a (DCP1A Human)
TagspAcGFP-C1ExpressionMammalianAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 ORF8
Plasmid#141278PurposeGateway-compatible Entry vector, with insert of ORF8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertORF8 (ORF8 SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
R4pGWB601_UBQ10p-Turbo-YFP-NLS
Plasmid#127368PurposeBinary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plantsDepositorInsertTurboID (BirA mutant)
TagsGS linker, NLS, V5, and YFPExpressionPlantMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only