315,318 results

1. pTRIPZ

   Plasmid

   #206981

   Purpose

   empty pTRIPZ vector for cloning of doxycycline-inducible gene expression

   Depositor

   Ming Chen

   Article

   Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SacI ML GFP Strand 11 Short

   Plasmid

   #164121

   Purpose

   Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

   Depositor

   Tito Calì, Marisa Brini

   Article

   Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

   Insert

   SacI ML GFP Strand 11 Short
   

   Expression

   Mammalian

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

   Plasmid

   #107941

   Purpose

   Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized enAsCas12a (E174R/S542R/K548R)
   

   Tags

   3x HA and NLS (nucleoplasmin)

   Expression

   Mammalian

   Mutation

   E174R, S542R and K548R

   Promoter

   CAG

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. NLS-LgBiT-FRB

   Plasmid

   #183403

   Purpose

   Expresses nuclear-localized LgBiT-FRB

   Depositor

   Alice Ting

   Article

   Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

   Insert

   LgBiT-FRB
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-HA-SLC38A9.1

   Plasmid

   #71869

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Jan 9;347(6218):188-94. do

   Insert

   SLC38A9.1 (SLC38A9 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA FLAG TORC2

   Plasmid

   #22975

   Depositor

   Marc Montminy

   Article

   Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

   Insert

   TORC2 (CRTC2 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA24N-p50-ligase

   Plasmid

   #87742

   Purpose

   IPTG-inducible expression of p50-ligase fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Tags

   6xHis and human p50/NF-kB (amino acids 40-366)

   Expression

   Bacterial

   Promoter

   T5-lac

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-EGFP-C1-Hs4E-T_AF

   Plasmid

   #148687

   Purpose

   Mammalian Expression of Hs4E-T

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

   Insert

   Hs4E-T (EIF4ENIF1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKS037 - pCAGGS-3XFLAG-Smc3-mKate2-bpA

   Plasmid

   #156447

   Purpose

   For transient expression of mouse SMC3 tagged with mKate2

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   mouse SMC3 (Smc3 Mouse)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCVpuro-Bcl2

    Plasmid

    #191964

    Purpose

    Exogenous expression of Bcl2 WT

    Depositor

    Luca Scorrano

    Insert

    Bcl2 (BCL2 Human)
    

    Use

    Retroviral

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PCMV-intron myc Rab2WT

    Plasmid

    #46779

    Purpose

    expression of WT Rab2a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB2 (RAB2A Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-GFP-IMP2-2

    Plasmid

    #42175

    Depositor

    Alexandra Kiemer

    Article

    Kessler et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Insulin-like growth factor 2 mRNA binding protein 2-2 (IGF2BP2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pUC18T-mini-Tn7T-Tp-ecfp

    Plasmid

    #65034

    Purpose

    TpR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    ecfp
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. V5-TurboID-Mfn1

    Plasmid

    #195155

    Purpose

    mammalian expression of TurboID tagged Mfn1

    Depositor

    Gia Voeltz

    Article

    Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

    Insert

    TurboID
    

    Tags

    Mfn1

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    CMV

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRIPZ (M)-HT-NLS

    Plasmid

    #82518

    Purpose

    Lentivirus vector. Expresses Halotag proteins with nuclear localization signal in mammalian cells.

    Depositor

    Xiaojun Ren

    Insert

    Nuclear localizaition sequence
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMV_AA050

    Plasmid

    #216107

    Purpose

    gDNA expression for Cas9 with ChenF+E trRNA (guide only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    gDNA expression for Cas9 with ChenF+E trRNA
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. px330-mcherry

    Plasmid

    #98750

    Purpose

    Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

    Depositor

    Jinsong Li

    Article

    Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. tetO.Nfib.Hygro

    Plasmid

    #117271

    Purpose

    Expresses Nfib and hygromycin resistance gene under control of tetO sequence.

    Depositor

    Henrik Ahlenius

    Article

    Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

    Insert

    Nfib (Nfib Mouse)
    

    Use

    Lentiviral

    Tags

    IRES and hygromycin resistant cassette (C termina…

    Expression

    Mammalian

    Promoter

    tet-on

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBtet-GN

    Plasmid

    #60501

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    TCE & RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV2-SynTetOff-palGFP

    Plasmid

    #190232

    Purpose

    AAV vector, high-level expression of EGFP with a palmitoylation signal derived from GAP-43 N-terminus (palGFP) in neuronal cells

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Insert

    EGFP
    

    Use

    AAV

    Tags

    palmitoylation signal sequence

    Expression

    Mammalian

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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