|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32752||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameμ2 subunit of clathrin adaptor complex AP-2
SpeciesR. norvegicus (rat)
Entrez GeneAp2m1 (a.k.a. Ap50, Clapm1, MGC105352, mu2)
- Promoter CMV
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
NB: The HA tag (YPYDVPDYALE) was inserted within the protein, between residues 236 and 237.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:μ2-HA-WT was a gift from Alexander Sorkin (Addgene plasmid # 32752 ; http://n2t.net/addgene:32752 ; RRID:Addgene_32752)
For your References section:Inhibition of the receptor-binding function of clathrin adaptor protein AP-2 by dominant-negative mutant mu2 subunit and its effects on endocytosis. Nesterov A, Carter RE, Sorkina T, Gill GN, Sorkin A. EMBO J. 1999 May 4;18(9):2489-99. 10.1093/emboj/18.9.2489 PubMed 10228163