μ2-HA-WT
(Plasmid #32752)

• Depositing Lab: Alexander Sorkin
• Publication: Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: μ2 subunit of clathrin adaptor complex AP-2
• Alt name: μ2
• Species: R. norvegicus (rat)
• Entrez Gene: Ap2m1 (a.k.a. Ap50, Clapm1, mu2)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: CMV-Fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: T. Kirchhausen, Harvard University
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NB: The HA tag (YPYDVPDYALE) was inserted within the protein, between residues 236 and 237.