|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27672||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5200
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
SpeciesR. norvegicus (rat)
Insert Size (bp)1400
Entrez GeneAp2m1 (a.k.a. Ap50, Clapm1, MGC105352, mu2)
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Rat μ2 was cloned from a construct provided by David Owen (see Collins et al. 2002).
mCherry was inserted within μ2 at residue 236 with BglII and NdeI sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AP2u2-mCherry was a gift from Christien Merrifield (Addgene plasmid # 27672 ; http://n2t.net/addgene:27672 ; RRID:Addgene_27672)
For your References section:A high precision survey of the molecular dynamics of Mammalian clathrin-mediated endocytosis. Taylor MJ, Perrais D, Merrifield CJ. PLoS Biol. 2011 Mar . 9(3):e1000604. 10.1371/journal.pbio.1000604 PubMed 21445324