AP2u2-mCherry
(Plasmid #27672)

• Depositing Lab: Christien Merrifield
• Publication: Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Backbone

• Vector backbone: pIRESneo3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ap2m1
• Alt name: mu2
• Species: R. norvegicus (rat)
• Insert Size (bp): 1400
• Entrez Gene: Ap2m1 (a.k.a. Ap50, Clapm1, mu2)
• Tag / Fusion Protein:
  • mCherry

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: IRES-R

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Rat μ2 was cloned from a construct provided by David Owen (see Collins et al. 2002).

mCherry was inserted within μ2 at residue 236 with BglII and NdeI sites.