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AP2u2-mCherry
(Plasmid #27672)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27672 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIRESneo3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ap2m1
  • Alt name
    mu2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1400
  • Entrez Gene
    Ap2m1 (a.k.a. Ap50, Clapm1, MGC105352, mu2)
  • Tag / Fusion Protein
    • mCherry

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Rat μ2 was cloned from a construct provided by David Owen (see Collins et al. 2002).

mCherry was inserted within μ2 at residue 236 with BglII and NdeI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AP2u2-mCherry was a gift from Christien Merrifield (Addgene plasmid # 27672 ; http://n2t.net/addgene:27672 ; RRID:Addgene_27672)
  • For your References section:

    A high precision survey of the molecular dynamics of Mammalian clathrin-mediated endocytosis. Taylor MJ, Perrais D, Merrifield CJ. PLoS Biol. 2011 Mar . 9(3):e1000604. 10.1371/journal.pbio.1000604 PubMed 21445324