165,888 results

1. pKI_ΩBBMVP16&WUS

   Plasmid

   #220348

   Purpose

   Expresses BBM-VP16 and WUS in plant cells

   Depositor

   Tomoko Igawa

   Article

   Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.

   Inserts

   BABY BOOM (BBM Mustard Weed)
   WUSCHEL (WUS Mustard Weed)
   

   Use

   Tags

   VP16 transcriptional activation domain

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S and RPS5A

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-cGAS-HA

   Plasmid

   #231895

   Purpose

   Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   cGAS (CGAS Human)
   

   Use

   Tags

   3xHA and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSC-NLS-RFP

   Plasmid

   #221813

   Purpose

   Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear localization sequence (NLS)

   Depositor

   Baojin Ding

   Article

   Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

   Insert

   NLS-mCherry
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-HisMBP

   Plasmid

   #11085

   Depositor

   David Waugh

   Article

   Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac (lactose/IPTG inducible)

   Available Since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. PDG458 V3

   Plasmid

   #226959

   Purpose

   CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6xHis-SNAP-PI(3)P Probe

   Plasmid

   #211508

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

   Depositor

   Hannes Maib, David Murray

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   FYVE-Hrs x 2 (PIKFYVE Human)
   

   Use

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57.1-Tet-UPF1WT (pRKB264)

   Plasmid

   #99146

   Purpose

   Inducible lentiviral construct to express FLAG-tagged UPF1wt

   Depositor

   Robert Bradley

   Article

   Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

   Insert

   UPF1 (UPF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   First 127 codons are optimized to reduce GC conte…

   Promoter

   pTight

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSBtet-Pur

   Plasmid

   #60507

   Purpose

   SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TCE & RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmiHiFiCas9

   Plasmid

   #164832

   Purpose

   miHiFiCas9 expressing plasmid

   Depositor

   Eugene Chen

   Article

   Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

   Insert

   miHiFiCas9 (contains Brex27 at sequence 5031-5138).
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Mammalian

   Mutation

   change R 691 to A

   Promoter

   CMV

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCK123 pDESTattB_exorh:mCerulean

    Plasmid

    #213512

    Purpose

    attB-containing pDEST plasmid, for use in Multisite Gateway reaction

    Depositor

    Christian Mosimann

    Article

    Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-G-Flamp1-mut

    Plasmid

    #188568

    Purpose

    control probe of G-Flamp1, insensitive to cAMP

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1-mut
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMAL-c2X_MBP-CLmN-H6

    Plasmid

    #234487

    Purpose

    Maltose binding protein N-terminally fused to His-tagged CLmN split intein

    Depositor

    Henning Mootz

    Article

    Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

    Insert

    N-terminal Fragment of the CLm Split Intein
    

    Use

    Tags

    Hexahistidine Tag and Maltose Binding Protein (MB…

    Expression

    Bacterial

    Mutation

    T69K, F75H, M118N within the N-terminal fragment …

    Promoter

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-PLE-IRES-mCherry

    Plasmid

    #45766

    Depositor

    Luke Lavis

    Article

    Tian et al Proc Natl Acad Sci U S A. 2012 Mar 27;10

    Insert

    esterase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. p18sheLL

    Plasmid

    #37321

    Depositor

    John Schiller

    Article

    Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

    Insert

    HPV18L1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpyTag003-Talin rod-mCherry

    Plasmid

    #133567

    Purpose

    Expresses SpyTag003-Talin rod-mCherry in mammalian cells

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-Talin rod-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. p7056 pHAGE-P-CMVt-N-HA-GAW-CPT1A

    Plasmid

    #100146

    Purpose

    Expresses CPT1A

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    CPT1A (CPT1A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVE13300

    Plasmid

    #137715

    Purpose

    Expresses impLbCas12a in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    impLbCas12a
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDY0663 ACTB atgRNA with v2 scaffold

    Plasmid

    #179108

    Purpose

    ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENN236_CRISPRcharm_Kv2

    Plasmid

    #220839

    Purpose

    CRISPRcharm Kv2 expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    CRISPRcharm Kv2
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPRi_Mxi1_yl

    Plasmid

    #91248

    Purpose

    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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