|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24541||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerGuntram Suske, Addgene plasmid 24544
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Gene/Insert nametranscription factor Sp3
SpeciesH. sapiens (human)
Insert Size (bp)2740
Entrez GeneSP3 (a.k.a. SPR2)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer AATCAACGGGACTTTCC, CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Two nucleotide mismatches with author sequence detected (one adjacent to the EcoRI cloning site and the second in the middle of the 5´-UTR). They do not affect the coding sequence of Sp3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pN3-Sp3FL was a gift from Guntram Suske (Addgene plasmid # 24541 ; http://n2t.net/addgene:24541 ; RRID:Addgene_24541)
For your References section:Complexity of translationally controlled transcription factor Sp3 isoform expression. Sapetschnig A, Koch F, Rischitor G, Mennenga T, Suske G. J Biol Chem. 2004 Oct 1. 279(40):42095-105. 10.1074/jbc.M404989200 PubMed 15247228