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pN3-Sp3FL
(Plasmid #24541)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24541 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pN3
  • Backbone manufacturer
    Guntram Suske, Addgene plasmid 24544
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    transcription factor Sp3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2740
  • Entrez Gene
    SP3 (a.k.a. SPR2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer AATCAACGGGACTTTCC, CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Two nucleotide mismatches with author sequence detected (one adjacent to the EcoRI cloning site and the second in the middle of the 5´-UTR). They do not affect the coding sequence of Sp3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pN3-Sp3FL was a gift from Guntram Suske (Addgene plasmid # 24541 ; http://n2t.net/addgene:24541 ; RRID:Addgene_24541)
  • For your References section:

    Complexity of translationally controlled transcription factor Sp3 isoform expression. Sapetschnig A, Koch F, Rischitor G, Mennenga T, Suske G. J Biol Chem. 2004 Oct 1. 279(40):42095-105. 10.1074/jbc.M404989200 PubMed 15247228