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pBXNPH3

Plasmid

#110098

Purpose

E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag and 3C cleavage site

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Tags

His

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBXNPH3

Plasmid

#110098

Purpose

E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag and 3C cleavage site

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Tags

His

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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p16sheLL

Plasmid

#37320

Depositor

John Schiller

Article

Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

Insert

HPV16L1+L2

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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p16sheLL

Plasmid

#37320

Depositor

John Schiller

Article

Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

Insert

HPV16L1+L2

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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SF-SON IRES Puro

Plasmid

#214402

Purpose

Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)

Depositor

Tanja Kuhlmann

Article

Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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SF-SON IRES Puro

Plasmid

#214402

Purpose

Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)

Depositor

Tanja Kuhlmann

Article

Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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DT480

Plasmid

#80413

Purpose

Expresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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DT480

Plasmid

#80413

Purpose

Expresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-3XFLAG-EGFP-OMP25

Plasmid

#83354

Purpose

For tagging mitochondria with Sigma FLAG epitopes

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XFLAG-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-3XFLAG-EGFP-OMP25

Plasmid

#83354

Purpose

For tagging mitochondria with Sigma FLAG epitopes

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XFLAG-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pFSW GFP IRES GFP

Plasmid

#133815

Purpose

Encodes EGFP that can be used as a control for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Type

Empty backbone

Use

Lentiviral

Tags

IRES-GFP

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pFSW GFP IRES GFP

Plasmid

#133815

Purpose

Encodes EGFP that can be used as a control for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Type

Empty backbone

Use

Lentiviral

Tags

IRES-GFP

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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DT240

Plasmid

#80414

Purpose

Expresses 240 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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DT240

Plasmid

#80414

Purpose

Expresses 240 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 neo

Plasmid

#98292

Purpose

Variant of lentiCRISPRv2 that confers G418 resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 neo

Plasmid

#98292

Purpose

Variant of lentiCRISPRv2 that confers G418 resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-NLRP3

Plasmid

#73955

Purpose

Express EGFP-tagged human NLRP3 in mammalian cells

Depositor

Christian Stehlik

Article

Khare et al Immunity. 2012 Mar 23;36(3):464-76. doi:

Insert

NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV iE

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-NLRP3

Plasmid

#73955

Purpose

Express EGFP-tagged human NLRP3 in mammalian cells

Depositor

Christian Stehlik

Article

Khare et al Immunity. 2012 Mar 23;36(3):464-76. doi:

Insert

NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV iE

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapFLEX.(mem).iGlucoSnFR2.mRuby3

Plasmid

#244101

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

IgG kappa leader and mRuby3

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pX330A-nBRD4/PITCh

Plasmid

#91794

Purpose

PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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