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pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (AAV Retrograde)

Viral Prep

#104491-AAVrg

Purpose

Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (#104491). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven, Cre-dependent, GCaMP7s calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Promoter

Syn

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only
pGP-AAV-syn-jGCaMP7b-WPRE (AAV1)

Viral Prep

#104489-AAV1

Purpose

Ready-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP7b-WPRE (#104489). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7b-WPRE plasmid DNA. Syn-driven jGCaMP7b calcium sensor. jGCaMP7b exhibits the brightest resting fluorescence of the jGCaMP7 variants, and can be used for imaging of small neuronal processes. These AAV preparations are suitable purity for injection into animals.

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Promoter

Syn

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only
pLentiPGK DEST H2B-iRFP670

Plasmid

#90237

Purpose

Lentiviral vector to express H2B-iRFP670 under PGK promoter

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiPGK DEST H2B-iRFP670

Plasmid

#90237

Purpose

Lentiviral vector to express H2B-iRFP670 under PGK promoter

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTP943

Plasmid

#104157

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fab specific nanobody TP886

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTP943

Plasmid

#104157

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fab specific nanobody TP886

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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TRE-KRAB-dCas9-IRES-GFP

Plasmid

#85556

Purpose

2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-GFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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TRE-KRAB-dCas9-IRES-GFP

Plasmid

#85556

Purpose

2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-GFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-AR V7

Plasmid

#86856

Purpose

Fluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)

Depositor

Michael Mancini, Marco Marcelli

Article

Szafran et al Prostate. 2017 Jan;77(1):82-93. doi: 10.

Insert

Androgen receptor (AR) (AR Human)

Tags

EGFP

Expression

Mammalian

Mutation

Alternative splice variant 7 (alteration/deletion…

Promoter

CMV

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-AR V7

Plasmid

#86856

Purpose

Fluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)

Depositor

Michael Mancini, Marco Marcelli

Article

Szafran et al Prostate. 2017 Jan;77(1):82-93. doi: 10.

Insert

Androgen receptor (AR) (AR Human)

Tags

EGFP

Expression

Mammalian

Mutation

Alternative splice variant 7 (alteration/deletion…

Promoter

CMV

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCuo CA Rac1

Plasmid

#84643

Purpose

constitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

Rac1 (RAC1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Constitutively Active, Q61L

Promoter

cumate-inducible promoter

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCuo CA Rac1

Plasmid

#84643

Purpose

constitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

Rac1 (RAC1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Constitutively Active, Q61L

Promoter

cumate-inducible promoter

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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Green cGull

Plasmid

#86867

Purpose

Green fluorescent probe for cGMP

Depositor

Tetsuya Kitaguchi

Article

Matsuda et al ACS Sens. 2017 Jan 27;2(1):46-51. doi: 1

Insert

Green cGull

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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Green cGull

Plasmid

#86867

Purpose

Green fluorescent probe for cGMP

Depositor

Tetsuya Kitaguchi

Article

Matsuda et al ACS Sens. 2017 Jan 27;2(1):46-51. doi: 1

Insert

Green cGull

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pKIR1.1

Plasmid

#85758

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG

Expression

Plant

Promoter

AtRPS5A

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pKIR1.1

Plasmid

#85758

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG

Expression

Plant

Promoter

AtRPS5A

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTRK669

Plasmid

#71313

Purpose

Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

Depositor

Rodolphe Barrangou, Todd Klaenhammer

Article

Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

Type

Empty backbone

Use

E. coli-gram positive bacteria shuttle vector

Expression

Bacterial

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pTRK669

Plasmid

#71313

Purpose

Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

Depositor

Rodolphe Barrangou, Todd Klaenhammer

Article

Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

Type

Empty backbone

Use

E. coli-gram positive bacteria shuttle vector

Expression

Bacterial

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.cTNT.iCre

Plasmid

#69916

Purpose

Used to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.

Depositor

William Pu

Article

Lin et al Circ Res. 2015 Jan 2;116(1):35-45. doi:

Has Service

AAV9

Insert

improved cre (iCre) recombinase and tdTomato

Use

AAV

Tags

The iCre coding frame and tdTomato coding frame i…

Promoter

Chicken cardiac troponin T

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.cTNT.iCre

Plasmid

#69916

Purpose

Used to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.

Depositor

William Pu

Article

Lin et al Circ Res. 2015 Jan 2;116(1):35-45. doi:

Has Service

AAV9

Insert

improved cre (iCre) recombinase and tdTomato

Use

AAV

Tags

The iCre coding frame and tdTomato coding frame i…

Promoter

Chicken cardiac troponin T

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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