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Addgene

pEGFP-C3-Exo70
(Plasmid #53761)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53761 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-C3
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6755
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Exo70
  • Alt name
    EXOC7
  • Alt name
    Exo70p
  • Alt name
    2-5-3p
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2055
  • GenBank ID
  • Entrez Gene
    EXOC7 (a.k.a. 2-5-3p, BLOM4, EX070, EXO70, EXOC1, Exo70p, NEDSEBA, YJL085W)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C3-Exo70 was a gift from Channing Der (Addgene plasmid # 53761 ; http://n2t.net/addgene:53761 ; RRID:Addgene_53761)
  • For your References section:

    Ral and Rheb GTPase activating proteins integrate mTOR and GTPase signaling in aging, autophagy, and tumor cell invasion. Martin TD, Chen XW, Kaplan RE, Saltiel AR, Walker CL, Reiner DJ, Der CJ. Mol Cell. 2014 Jan 23;53(2):209-20. doi: 10.1016/j.molcel.2013.12.004. Epub 2014 Jan 2. 10.1016/j.molcel.2013.12.004 PubMed 24389102