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pEGFP-C3-Sec8
(Plasmid #53758)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53758 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C3
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7626
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sec8
  • Alt name
    EXOC4
  • Alt name
    SEC8P
  • Alt name
    SEC8L1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2926
  • GenBank ID
  • Entrez Gene
    EXOC4 (a.k.a. SEC8, SEC8L1, Sec8p)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C3-Sec8 was a gift from Channing Der (Addgene plasmid # 53758 ; http://n2t.net/addgene:53758 ; RRID:Addgene_53758)
  • For your References section:

    Ral and Rheb GTPase activating proteins integrate mTOR and GTPase signaling in aging, autophagy, and tumor cell invasion. Martin TD, Chen XW, Kaplan RE, Saltiel AR, Walker CL, Reiner DJ, Der CJ. Mol Cell. 2014 Jan 23;53(2):209-20. doi: 10.1016/j.molcel.2013.12.004. Epub 2014 Jan 2. 10.1016/j.molcel.2013.12.004 PubMed 24389102