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pMDC32B-AtMIR390a-B/c
(Plasmid #51776)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51776 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMDC32
  • Backbone size w/o insert (bp) 10133
  • Total vector size (bp) 12044
  • Modifications to backbone
    A BsaI site of the backbone was mutated to block the site.
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    E. coli DB3.1 (or other strain resistant to ccdB) at 37C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AtMIR390a-B/c
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1911
  • Mutation
    A. thaliana MIR390a precursor sequence including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites. The BsaI site in the ccdB gene was mutated to block the site.

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMDC32B-AtMIR390a-B/c was a gift from James Carrington (Addgene plasmid # 51776)
  • For your References section:

    New Generation of Artificial MicroRNA and Synthetic Trans-Acting Small Interfering RNA Vectors for Efficient Gene Silencing in Arabidopsis. Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, Carrington JC. Plant Physiol. 2014 Mar 19. 10.1104/pp.113.234989 PubMed 24647477