|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51776||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 10133
- Total vector size (bp) 12044
Modifications to backboneA BsaI site of the backbone was mutated to block the site.
Vector typePlant Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Kanamycin
Growth instructionsE. coli DB3.1 (or other strain resistant to ccdB) at 37C
Copy numberHigh Copy
SpeciesA. thaliana (mustard weed)
Insert Size (bp)1911
MutationA. thaliana MIR390a precursor sequence including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites. The BsaI site in the ccdB gene was mutated to block the site.
- Cloning method Unknown
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13-Rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMDC32B-AtMIR390a-B/c was a gift from James Carrington (Addgene plasmid # 51776)
For your References section:New Generation of Artificial MicroRNA and Synthetic Trans-Acting Small Interfering RNA Vectors for Efficient Gene Silencing in Arabidopsis. Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, Carrington JC. Plant Physiol. 2014 Mar 19. 10.1104/pp.113.234989 PubMed 24647477