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pCDHblast MCSNard OST-LMNAd50
(Plasmid #22662)


Item Catalog # Description Quantity Price (USD)
Plasmid 22662 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    LaminAd50 (=Progerin)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    50AA deletion creating Progerin instead of mature LMNA
  • Tag / Fusion Protein
    • Onestrep Tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI, XhoI, NotI, SwaI, BglII, AsuII (not destroyed)
  • 5′ sequencing primer AAATGGGCGGTAGGCGTGT
  • 3′ sequencing primer TCTCTAGGCACCCGTTCAAT
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDHblast MCSNard OST-LMNAd50 was a gift from Tom Misteli (Addgene plasmid # 22662 ; ; RRID:Addgene_22662)
  • For your References section:

    Ageing-related chromatin defects through loss of the NURD complex. Pegoraro G, Kubben N, Wickert U, Göhler H, Hoffmann K, Misteli T. Nat Cell Biol. 2009 Oct . 11(10):1261-7. 10.1038/ncb1971 PubMed 19734887