|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17663||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5200
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameGFP-D50 lamin A
Alt namelamin A
SpeciesH. sapiens (human)
Entrez GeneLMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
/ Fusion Protein
- EGFP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This vector was generated by cloning the NheI–BamHI fragment from pEGFP–D50 lamin A into the BamHI site of the pBABE-puro vector after filling of the ends.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBABE-puro-GFP-progerin was a gift from Tom Misteli (Addgene plasmid # 17663 ; http://n2t.net/addgene:17663 ; RRID:Addgene_17663)
For your References section:Lamin A-dependent misregulation of adult stem cells associated with accelerated ageing. Scaffidi P, Misteli T. Nat Cell Biol. 2008 Mar 2. ():. 10.1038/ncb1708 PubMed 18311132