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pRN11
(Plasmid #84455)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84455 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCM29
  • Backbone size w/o insert (bp) 6604
  • Total vector size (bp) 6574
  • Modifications to backbone
    sGFP replaced with insert
  • Vector type
    shuttle vector E.coli-S.aureus, expression of sGFP from the SarA-P1 promoter
  • Selectable markers
    also contains Chloramphenicol resistance gene, but only functional in Gram-postive bacteria.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E.coli DC10b (described in doi: 10.1128/mBio.00537-12)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    739

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site xmai (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TTGCATGCCTGCAGGTCGACTCTA
  • 3′ sequencing primer TTATGCTTCCGGCTCGTATGTTGTGTGG
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    mCherry amplified from pJL49 ; Liese J, Rooijakkers SHM, van Strijp JAG, Novick RP, Dustin ML. 2013. Intravital two-photon microscopy of host–pathogen interactions in a mouse model of Staphylococcus aureus skin abscess formation. Cellular microbiology 15:891.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRN11 was a gift from Reindert Nijland (Addgene plasmid # 84455 ; http://n2t.net/addgene:84455 ; RRID:Addgene_84455)
  • For your References section:

    Fluorescent reporters for markerless genomic integration in Staphylococcus aureus. de Jong NW, van der Horst T, van Strijp JA, Nijland R. Sci Rep. 2017 Mar 7;7:43889. doi: 10.1038/srep43889. 10.1038/srep43889 PubMed 28266573