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pcDNA GNSTM-3-Flag-10-Lamp2b-HA
(Plasmid #71293)


Item Catalog # Description Quantity Price (USD)
Plasmid 71293 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pcDNA 3.1+ Hygro
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5578
  • Total vector size (bp) 6925
  • Modifications to backbone
    some restriction sites removed from MCS
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Entrez Gene
    LAMP2 (a.k.a. CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Lamp2 signal peptide (N terminal on insert)
    • GNSTM glycosylation motif (N terminal on insert)
    • FLAG (N terminal on insert)
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Lamp2b cDNA was purchased from Open Biosystems
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA GNSTM-3-Flag-10-Lamp2b-HA was a gift from Joshua Leonard (Addgene plasmid # 71293 ; ; RRID:Addgene_71293)
  • For your References section:

    Stabilization of exosome-targeting peptides via engineered glycosylation. Hung ME, Leonard JN. J Biol Chem. 2015 Mar 27;290(13):8166-72. doi: 10.1074/jbc.M114.621383. Epub 2015 Feb 5. 10.1074/jbc.M114.621383 PubMed 25657008