We narrowed to 1,086 results for: gibson assembly

1. pBIG1c

   Plasmid

   #80613

   Purpose

   pBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBIG1d

   Plasmid

   #80614

   Purpose

   pBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBIG1e

   Plasmid

   #80615

   Purpose

   pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. STAGR_gRNAScaffold_mU6

   Plasmid

   #102844

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert gRNAScaffold_mU6
   

   Use

   Pcr template for stagr inserts

   Promoter

   mU6

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mCherry-Dyskerin

   Plasmid

   #207577

   Purpose

   N-terminal mCherry-tag using SalI/EcoRI

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   generated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. STAGR_gRNAScaffold_hH1

   Plasmid

   #102841

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert gRNAScaffold_hH1
   

   Use

   Pcr template for stagr inserts

   Promoter

   hH1

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 3xFLAG-Halo-Dyskerin

   Plasmid

   #207578

   Purpose

   CRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   HaloTag DKC1
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGK:HygroR-CMV:mKate2-B_UTR

   Plasmid

   #68480

   Purpose

   Lentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Hygromycin Resistance
   mKate2-B_UTR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   shRNA sensor cassette "B" added to 3&#x…

   Promoter

   CMV and pGK

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. STAgR_Neo

   Plasmid

   #102992

   Purpose

   Can be used as backbone for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Type

   Empty backbone

   Use

   CRISPR; Pcr template for stagr vectors

   Promoter

   U6

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Halo-TCAB1 HRD

    Plasmid

    #207535

    Purpose

    Homologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    HaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. STAgR_tdTomato

    Plasmid

    #102993

    Purpose

    Can be used as backbone for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Type

    Empty backbone

    Use

    CRISPR; Pcr template for stagr vectors

    Promoter

    U6

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGK:HygroR-CMV:mTagBFP2-A_UTR

    Plasmid

    #68479

    Purpose

    Lentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistance

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    Hygromycin Resistance
    mTagBFP2-A_UTR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    shRNA sensor cassette "A" added to 3&#x…

    Promoter

    CMV and pGK

    Available Since

    Nov. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBTK599

    Plasmid

    #110600

    Purpose

    BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP dropout
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pL0_AC-CEN_ARS_HIS

    Plasmid

    #202129

    Purpose

    Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    CEN_ARS_HIS
    

    Use

    Synthetic Biology

    Mutation

    ARS sequence mutated to remove sapI site for dome…

    Available Since

    Aug. 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pGK:TRL-EFS:iRFP670

    Plasmid

    #68475

    Purpose

    Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    tTR-KRAB-2A-rtTA3-2A-Luciferase
    iRFP670
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    EFS and pGK

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CAG-miniSOG

    Plasmid

    #193913

    Purpose

    Control plasmid that expresses non-targeted miniSOG in mammalian cells

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pL0_EF-PtBle

    Plasmid

    #202180

    Purpose

    Level 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    ShBle expression cassette
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. TOPO rtTA3-2A-Cre

    Plasmid

    #68448

    Purpose

    TOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    rtTA3-2A-Cre
    

    Use

    Gmap

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in mice

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. DEP-GFP

    Plasmid

    #109043

    Purpose

    Expression of the Dishevelled DEP domain

    Depositor

    Mariann Bienz

    Article

    Gammons et al Mol Cell. 2016 Oct 6;64(1):92-104. doi:

    Insert

    dishevelled segment polarity protein 2 (DVL2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Dvl2 DEP domain

    Promoter

    CMV

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

    Plasmid

    #172504

    Purpose

    Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge