We narrowed to 7,278 results for: gan
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Plasmid#223337PurposeMapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorInsertABCD1-TurboID-EGFP-3HA (PXA1 Mustard Weed)
TagsTurboID-EGFP-3HAExpressionPlantPromoterCaMV35SAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-NRG1-ScNeo
Plasmid#209911PurposeTo monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEndo-I-OnuI-TSM
Plasmid#207954PurposeExpresses a fusion of the homing endonuclease I-OnuI with the thermosensitive L212P VMA1 intein. Endonuclease activity is restricted to lower temperatures.DepositorInsertThermosensitive fusion of I-OnuI and the L212P VMA1 intein.
UseSynthetic BiologyExpressionBacterialMutationLeucine 212 changed to proline, leucine 434 in fu…Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-avi-TEV-LIC_DCAF12:1-453
Plasmid#210940PurposeInsect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.DepositorInsertDCAF12:M1-S453 (DCAF12 Human)
ExpressionInsectMutationR131Q natural variant (VAR_035322)PromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_WDR19:1-636
Plasmid#210933PurposeInsect expression of human WDR19 fragment M1 to G636. N-terminal 6X His tag with TEV cleavage.DepositorInsertWDR19:M1-G636 (WDR19 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBiT3.1-N_LRRK2:2142-2527
Plasmid#211112PurposeMammalian expression of human LRRK2 fragment R2142 to E2527. N-terminal HiBiT tag.DepositorInsertLRRK2:R2142-E2527 (LRRK2 Human)
TagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationM2397T natural variant (VAR_024965)PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_EIF3B:186-685
Plasmid#211205PurposeMammalian expression of human EIF3B fragment V186 to T685. N-terminal HiBiT tag.DepositorInsertEIF3B:V186-T685 (EIF3B Human)
TagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_LRRK2:2142-2527
Plasmid#211151PurposeMammalian expression of human LRRK2 fragment R2142 to E2527. C-terminal HiBiT tag.DepositorInsertLRRK2:R2142-E2527 (LRRK2 Human)
TagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationM2397T natural variant ( VAR_024965)PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_LRRK2:2141-2527
Plasmid#210901PurposeInsect expression of human LRRK2 fragment T2141 to E2527. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertLRRK2:T2141-E2527 (LRRK2 Human)
TagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationM2397T natural variant ( VAR_024965)PromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBiT3.1-C_EIF3B:186-685
Plasmid#211279PurposeMammalian expression of human EIF3B fragment V186 to T685. C-terminal HiBiT tag.DepositorInsertEIF3B:V186-T685 (EIF3B Human)
TagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_DNAI4:1-848
Plasmid#211258PurposeMammalian expression of full-length human DNAI4. N-terminal HiBiT tag.DepositorInsertDNAI4:M1-A848 (WDR78 Human)
TagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationG16A natural variant VAR_057635. C33W natural v…PromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS
Plasmid#194753PurposeLentiviral expression of BioID2-HA-13 x Linker- RBP(V) on the cell surface via a GPI anchorDepositorInsertPrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS
UseLentiviralTagsHAPromoterCMVAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PrP (1-22)-RBP(V)-13 x Linker-BioID2-HA-GPI-PSS
Plasmid#194752PurposeLentiviral expression of RBP(V)-13 x Linker-BioID2-HA on the cell surface via a GPI anchorDepositorInsertPrP (1-22)-RBP(V)-13 x Linker-BioID2-HA-GPI-PSS
UseLentiviralTagsHAPromoterCMVAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
PABPC1_544-636_pGEX6P1
Plasmid#209789PurposeBacterial expression of PABPC1 Mlle domainDepositorInsertMlle domain of PABPC1 (PABPC1 Human)
TagsGSTExpressionBacterialMutationaa 544-636 onlyPromotertacAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 AURKA deltaNter
Plasmid#157749PurposeExpression of AURKA without Nter in mammalian cellsDepositorInsertAURKA (AURKA Human)
ExpressionMammalianMutationthe first 30AA of Aurora A are lackingPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EEF1A2_GTP-EFTU-D2
Plasmid#109919PurposeProtein expression and purification of EEF1A2_GTP-EFTU-D2DepositorAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-H253A-R256A-R260A
Plasmid#127788PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in His2…Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2
Plasmid#84719PurposeE. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcriptionDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
ExpressionBacterialMutation12 nt stretch lacking A's inserted before 5&…Available sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-mEGFP
Plasmid#105143PurposeYeast gene targetingDepositorInsertkanMX6-P81nmt1-mEGFP
Tags81nmt1-mEGFPExpressionBacterial and YeastMutationA206KPromoter81nmt1Available sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
C21-bio-His
Plasmid#53405PurposeExpresses full-length ES1 protein homolog precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertC21 (GATD3 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only