We narrowed to 1,810 results for: gibson assembly

1. pBN084

   Plasmid

   #173446

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with Y708E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with Y708E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, Y708E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBN085

   Plasmid

   #173447

   Purpose

   express E. coli E69 (O9a:K30) Wzc_K540M with Y713E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc_K540M with Y713E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   K540M, Y713E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBN079

   Plasmid

   #167960

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y718E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y718E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y718E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBN080

   Plasmid

   #167961

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y717E, Y718E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y717E, Y718E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y717E, Y718E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBN072

   Plasmid

   #167953

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y705E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y705E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y705E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBN073

   Plasmid

   #167954

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y706E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y706E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y706E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBN074

   Plasmid

   #167955

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y705E, Y706E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y705E, Y706E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y705E, Y706E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBN075

   Plasmid

   #167956

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y708E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y708E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y708E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBN076

   Plasmid

   #167957

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y713E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y713E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y713E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBN077

    Plasmid

    #167958

    Purpose

    express E. coli E69 (O9a:K30) Wzc with Y715E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with Y715E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Y715E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBN078

    Plasmid

    #167959

    Purpose

    express E. coli E69 (O9a:K30) Wzc with Y717E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with Y717E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Y717E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AP1298‐1

    Plasmid

    #87248

    Purpose

    gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

    Insert

    gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. C79m

    Plasmid

    #120991

    Purpose

    MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    ccdA antitoxin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. V37m

    Plasmid

    #121043

    Purpose

    MoClo golden gate assembly AE destination vector DVCK-AE-mScar (ColE1/kan resistance destination vector with constitutive mScarlet expressing for screening.

    Depositor

    Richard Murray

    Insert

    Kan destination vector with ColE1 origin and mScarlet selection
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C83m

    Plasmid

    #120994

    Purpose

    MoClo golden gate assembly CD part for pir (Initiation factor required for replication of R6K origin plasmids). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    pir protein required for R6K ori
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. C24m

    Plasmid

    #120964

    Purpose

    MoClo golden gate assembly CD part for lambda cI (repressor of pL or pOR1-OR2; use C64m for temperature-sensitive version). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    lambda-cI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. C27m

    Plasmid

    #120965

    Purpose

    MoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    tetR (no deg tags)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C98m

    Plasmid

    #121002

    Purpose

    MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    Gp2 toxin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. C51m

    Plasmid

    #120975

    Purpose

    MoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank file

    Depositor

    Richard Murray

    Insert

    sfYFP (has BbsI site)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. C33m

    Plasmid

    #120968

    Purpose

    MoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    AiiA degradase (Bennett, no ssra tag)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge