Addgene

We narrowed to 1,014 results for: gibson assembly

Showing: 701 - 720 of 1014 results

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pL0_CD-eCFP

Plasmid

#202142

Purpose

Level 0 plasmid containing an eCFP fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

eCFP gene

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_CD-greenlantern

Plasmid

#202141

Purpose

Level 0 plasmid containing a green lantern fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Green lantern gene

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_CD-roGFP

Plasmid

#202140

Purpose

Level 0 plasmid containing a roGFP fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

roGFP gene

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_CD-eYFP

Plasmid

#202139

Purpose

Level 0 plasmid containing an eYFP fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

eYFP gene

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_CD-PT2A

Plasmid

#202138

Purpose

Level 0 plasmid containing a dual 2A (P2A and T2A) peptide sequence with CD overhangs used to build level 1 constructs

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Dual 2A peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_CD-GUS

Plasmid

#202137

Purpose

Level 0 plasmid containing a beta glucuronidase reporter gene with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Beta glucuronidase gene

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_BC-ER_sig_pep

Plasmid

#202136

Purpose

Level 0 plasmid containing a kozak consensus sequence and a ER signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Endoplasmic reticulum signal peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-nuc_sig_pep

Plasmid

#202135

Purpose

Level 0 plasmid containing a kozak consensus sequence and a nucleus signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Nucleus signal peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-mito_sig_pep

Plasmid

#202134

Purpose

Level 0 plasmid containing a kozak consensus sequence and a mitochondria signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Mitochondria signal peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-sec_sig_pep

Plasmid

#202133

Purpose

Level 0 plasmid containing a kozak consensus sequence and a secretion signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Secretion signal peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_BC-chl_sig_pep

Plasmid

#202131

Purpose

Level 0 plasmid containing a kozak consensus sequence and a chloroplast signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Chloroplast signal peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AF-spacer

Plasmid

#202130

Purpose

Level 0 plasmid containing a small non-coding spacer sequence with AF overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Spacer

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-acc2

Plasmid

#202128

Purpose

Level 0 plasmid containing the promoter from gene 55209 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

acc2 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_AC-glna

Plasmid

#202127

Purpose

Level 0 plasmid containing the promoter from gene 22357 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

glna promoter

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-dph1

Plasmid

#202126

Purpose

Level 0 plasmid containing the promoter from gene 54330 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

dph1 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-NR

Plasmid

#202123

Purpose

Level 0 plasmid containing the promoter from gene 54983 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NR promoter

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_AC-fcpB

Plasmid

#202121

Purpose

Level 0 plasmid containing the promoter from gene 25172 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

fcpB promoter

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_AC-vapol

Plasmid

#202118

Purpose

Level 0 plasmid containing the promoter from gene 47434 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

vapol promoter

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_AC-lysA

Plasmid

#202117

Purpose

Level 0 plasmid containing the promoter from gene 21592 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

lysA promoter

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_AC-HO

Plasmid

#202116

Purpose

Level 0 plasmid containing the promoter from gene 5902 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

HO promoter

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge