We narrowed to 911 results for: pgem

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Hox B8a

Plasmid

#36862

Depositor

Robert Ho

Article

Prince et al Development. 1998 Feb . 125(3):407-20.

Insert

Hox B8a (hoxb8a Zebrafish)

Use

Riboprobe synthesis

Expression

Bacterial

Mutation

D238E

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pmTgfa UTR

Plasmid

#31256

Depositor

David Lee

Article

Kornblum et al J Comp Neurol. 1997 Apr 7. 380(2):243-61

Insert

Tgfa (Tgfa Mouse)

Use

Cdna for in situ hybridization

Mutation

Exon 6 of Tgfa with 3'UTR

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only
p-rlf-1

Plasmid

#14056

Depositor

Richard Hynes

Article

Schwarzbauer et al Cell. 1983 Dec . 35(2 Pt 1):421-31.

Insert

Rat Fibronectin (Fn1 Rat)

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pcFNE3A (chick fibronectin E3A)

Plasmid

#14062

Depositor

Richard Hynes

Article

Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

Insert

Chicken Fibronectin EIIIA (FN1 Chicken)

Expression

Mammalian

Mutation

Blunted BstE2-AvaII fragment was inserted into bl…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
CRISPR-Cas9 Gateway System for Physcomitrella patens

Plasmid Kit

#1000000151

Purpose

CRISPR-Cas9 Gateway System is a collection of 24 plasmids providing a modular, efficient CRISPR/Cas9 editing system for accurate and customizable repair in Physcomitrella patens.

Depositor

Magdalena Bezanilla

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only
prDNA spacer-LacOx120-20xMS2-Hyg

Plasmid

#210220

Purpose

Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

120xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pISH-Dlx6

Plasmid

#74345

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx6. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

distal-less homeobox 6 (Dlx6 Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pEn-Chimera

Plasmid

#61432

Purpose

Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

AtU6-26:sgRNA

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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HBV 1.3-mer P-null replicon

Plasmid

#65462

Purpose

containing 1.3 units of the P-null HBV genome (subtype ayw)

Depositor

Wang-Shick Ryu

Article

Wang et al J Virol. 2009 Jun;83(11):5815-24. doi: 1

Insert

1.3 units of the P-null HBV genome

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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prDNA spacer-LacOx50-20xMS2-Hyg

Plasmid

#210219

Purpose

Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pISH-Pax6

Plasmid

#74350

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pax6. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

paired box 6 (Pax6 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pSLI-TGD(1463000)

Plasmid

#85791

Purpose

P. falciparum integration plasmid to disrupt genes using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PF3D7146300 targeting region, GFP-2TA-Neo-R

Use

P. falciparum genomic integration (gene disruptio…

Tags

GFP

Promoter

none

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Cux1

Plasmid

#74341

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Cux1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

cut-like homeobox 1 (Cux1 Mouse)

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMH-Cas9-gate

Plasmid

#113742

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR; Gateway destination vector

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSLI-2xFKBP-GFP(1463000)

Plasmid

#85789

Purpose

P. falciparum integration plasmid to fuse endogenous genes with tags using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PF3D7146300 targeting region, 2xFKBP-GFP-2TA-Neo-R

Use

P. falciparum integration

Tags

2xFKBP, GFP

Promoter

none

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pSLI-sandwich(0807600)

Plasmid

#85790

Purpose

P. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R

Use

P. falciparum genomic integration

Tags

2xFKBP, GFP

Promoter

none

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pGolf-ISH

Plasmid

#15853

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

Guanine Nucleotide Binding Protein, Alpha Stimulating, Olfactory type (Gnal Mouse)

Use

Cloning vector

Mutation

F199S

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pFH6

Plasmid

#86555

Purpose

Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency

Depositor

Andreas Weber

Article

Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

Insert

U6-26p::sgRNA scaffold

Use

CRISPR; Subcloning

Promoter

Arabidopsis U6-26 promoter

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pSLI-N-GFP-2xFKBP-loxP(K13)

Plasmid

#85792

Purpose

P. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

Kelch13

Use

Cre/Lox; P. falciparum genomic integration

Tags

2xFKBP, GFP

Promoter

none

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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Hox D4a

Plasmid

#36469

Depositor

Robert Ho

Article

Prince et al Development. 1998 Feb . 125(3):407-20.

Insert

Hox D4a (hoxd4a Zebrafish)

Use

Riboprobe synthesis

Expression

Bacterial

Mutation

Q100H, A101T, G125C and R189W

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only