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Showing: 7221 - 7232 of 7232 results

  1. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

    Plasmid
    #202554
    Purpose
    Synaptotagmin-1 sgRNA1 with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202554-plasmid-map-sequence-id-404687

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  2. pDual

    Plasmid
    #207883
    Purpose
    Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.
    Depositor
    David Edgell
    Article
    Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206
    Inserts
    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Leucine 212 changed to proline, leucine 434 in th…
    Available Since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207883-plasmid-map-sequence-id-422555

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  3. pVZ_PcoaT_SQE1_THAS1

    Plasmid
    #103859
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and THAS1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    thalianol synthase 1 (THAS1 Mustard Weed)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available Since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    103859-plasmid-map-sequence-id-217087

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  6. cAMP-dependent protein kinase (PKA) regulatory 1a subunit (R1a)

    Plasmid
    #215436
    Purpose
    Bacterially expressed R1a (no HiBiT) Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Human cAMP-dependent protein kinase type I-alpha subunit (PRKAR1A) mRNA, complete cds, C-terminal 10X-His.Thrombin Tag
    Tags
    10X His-tag with thrombin site
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available Since
    June 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    215436-plasmid-map-sequence-id-428696

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  8. pT22iuasvApoWChe(#488)

    Plasmid
    #184108
    Purpose
    cyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
    Use
    Zebrafish transgenesis (blue eyes)
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    4xUAS
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184108-plasmid-map-sequence-id-363970

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  9. pT2iApowriter2(#336)

    Plasmid
    #184104
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transient transgenesis
    Tags
    internal tag: 5myc
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184104-plasmid-map-sequence-id-364017

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  10. pT2iU6Apowriter2N(#355)

    Plasmid
    #184106
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transient transgenesis + in vitro trans…
    Tags
    internal tag: 5myc
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi+SP6
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184106-plasmid-map-sequence-id-364015

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  11. pT22Apowriter2(#340)

    Plasmid
    #184105
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    internal tag: 5myc
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184105-plasmid-map-sequence-id-363997

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  12. pT22iuasvApoWtEos3N(#486)

    Plasmid
    #184107
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transgenesis (blue eyes)
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    4xUAS
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184107-plasmid-map-sequence-id-363989

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Showing: 7221 - 7232 of 7232 results