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pHH0103_S100A11_S-100

Plasmid

#109804

Purpose

Protein expression and purification of S100A11_S-100

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

S100A11_S-100 (S100A11 Human)

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA_DiCre_59.F2

Plasmid

#60207

Purpose

Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Bacteriophage P1, Human, Synthetic)

Tags

FKBP12 and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 19-59 of the humanized version of Cre…

Promoter

CMV

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Sec61B

Plasmid

#120244

Purpose

Expresses V5-tagged Sec61B in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Sec61B (Sec61b Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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ZJOM141

Plasmid

#133669

Purpose

Integration of COX9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-COX9-mTagBFP2 (COX9 Budding Yeast)

Tags

mTagBFP2

Expression

Yeast

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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CFP-GAI(1-532)

Plasmid

#37310

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Tags

CFP

Expression

Mammalian

Mutation

contains aa 1-532

Promoter

CMV

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
CSII-IDR2-owlTRIMCyp-FOS

Plasmid

#79067

Purpose

Express owl monkey TRIMCyp in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5Cyp (owl monkey)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET-17b_Unc104-KIF5B-HALO

Plasmid

#169996

Purpose

Ultra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labeling

Depositor

Ron Vale

Article

Niekamp et al Nat Methods. 2020 Apr;17(4):437-441. doi

Insert

Kinesin-like protein Unc-104 fused with KIF5B

Tags

HALO-tag, 6xHIS

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMOD_C2813

Plasmid

#91092

Purpose

Module C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4 (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

PvUbi2

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pXPR_003 Intergenic control guide 2

Plasmid

#193585

Purpose

sgRNA control; induces CAS9 cutting in an intergenic region

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgRNA control

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgNFkB2 guide 2

Plasmid

#193594

Purpose

NFkB2 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgNFkB2 guide 2 (NFKB2 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR-Kras

Plasmid

#118408

Purpose

Plasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR-Kras

Tags

C-terminal targeting sequence from Kras

Expression

Mammalian

Promoter

CMV

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_017

Plasmid

#123932

Purpose

Encodes the BxBI attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BxBI attB tagBFP destination vector

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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S12.1_Empty_Bridge

Plasmid

#127670

Purpose

Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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NELFE-mOrange pHis-//

Plasmid

#53303

Purpose

Expresses His-tagged NELF-E-mOrange fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

NELF-E protein (Nelf-E Fly)

Tags

His and mOrange

Expression

Bacterial

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2mFoxd3

Plasmid

#37267

Depositor

Patricia Labosky

Article

Steiner et al Development. 2006 Dec;133(24):4827-38. E

Insert

Foxd3 (Foxd3 Mouse)

Use

Overexpression

Expression

Mammalian

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Nxph4-HA

Plasmid

#128102

Purpose

Express HA tagged Nxph4

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nxph4 (Nxph4 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_B2517

Plasmid

#91074

Purpose

Module B, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

TaU3

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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ZJOM2

Plasmid

#133633

Purpose

Integration of VRG4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-VRG4-GFP (VRG4 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40

Plasmid

#112496

Purpose

Expresses channel rhodopsin in astrocytes

Depositor

Baljit Khakh

Article

Octeau et al Cell Rep. 2019 May 21;27(8):2249-2261.e7

Insert

ChR2(H134R)

Use

AAV

Tags

mCherry

Mutation

H134R

Promoter

GfaABC1D

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pUSE-Src-KD-UniRapR-mCerulean-myc

Plasmid

#45382

Depositor

Klaus Hahn

Article

Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

Src-KD-UniRapR-mCerulean-myc

Tags

mCerulean and myc

Expression

Mammalian

Mutation

mSrc R388D (kinase dead), Y529F

Promoter

CMV

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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