We narrowed to 5,561 results for: donor

1. pDD362

   Plasmid

   #137023

   Purpose

   SapTrap HaloTag-GLO donor

   Depositor

   Bob Goldstein, Daniel Dickinson

   Insert

   C. elegans GLO-optimized Halo Tag
   

   Use

   CRISPR; Saptrap donor for cloning repair templat…

   Tags

   C. elegans GLO-optimized Halo Tag

   Expression

   Worm

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-f1(+)-ACTB-mEGFP

   Plasmid

   #115909

   Purpose

   donor to insert EGFP at the ACTB locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   ACTB-mEGFP (ACTB Human)
   

   Use

   Donor template

   Tags

   EGFP

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-f1(+)-ACTB-iTagRFP-T

   Plasmid

   #115915

   Purpose

   donor to insert iTagRFP-T at the ACTB locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   ACTB-TagRFP-T (ACTB Human)
   

   Use

   Donor template

   Tags

   TagRFP-T

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNP1954

   Plasmid

   #222730

   Purpose

   pNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 bridge RNA
   

   Tags

   sfGFP

   Expression

   Bacterial

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Intron-Tagging-pX330-Cas9-Blast

   Plasmid

   #159741

   Purpose

   Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   donor plasmid targeting sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-ACTB-C200-GFPfr1

   Plasmid

   #169798

   Purpose

   THe donor vector for the ACTB gene locus.

   Depositor

   Natsuko Chiba

   Article

   Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

   Insert

   Donor sequence to ACTB gene for HR
   

   Use

   Hr donor vector for human actb gene.

   Mutation

   Partial sequence of GFP is inserted before the st…

   Available Since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-KLF7-200-GFPfr1

   Plasmid

   #169799

   Purpose

   THe donor vector for the KLF7 gene locus.

   Depositor

   Natsuko Chiba

   Article

   Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

   Insert

   Donor sequence to KLF7 neighbouring region for HR
   

   Use

   Hr donor vector for human actb gene.

   Mutation

   Partial sequence of GFP is inserted the sequence …

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Intron-Tagging-pX330-Cas9-mCherry

   Plasmid

   #159742

   Purpose

   Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   donor plasmid targeting sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPaint-T2A-LexGAD-3xP3-RFP

   Plasmid

   #127558

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   LexGAD
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

    Plasmid

    #175573

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

    Plasmid

    #175574

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Anti-MAPT [AT8] (Recombinant Antibody)

    Antibody

    #216150-rAb

    Purpose

    Anti-MAPT (Human) recombinant mouse monoclonal antibody; recognizes the disease-associated version of Tau (phospho-Tau)

    Depositor

    Melina Fan

    Article

    Mercken et al Acta Neuropathol. 1992;84(3):265-72. doi

    Recommended Applications

    Immunohistochemistry

    Reactivity

    Human

    Source Species

    Mouse

    Isotype

    IgG2a

    Trial Size

    Not available to purchase

    Available Since

    March 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

13. pBS-ACTB-C200-T7

    Plasmid

    #229850

    Purpose

    Donor vector for the ACTB site containing T7 sequence for detection of HR products.

    Depositor

    Natsuko Chiba

    Insert

    Partial sequence of ACTB gene
    

    Use

    Hr donor vector for human actb gene.

    Available Since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pscAAV-Tyr

    Plasmid

    #135616

    Purpose

    Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    EcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP

    Plasmid

    #127560

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Cas9-T2A-GFP
    

    Use

    CRISPR

    Expression

    Insect

    Mutation

    EGFP-D102G see depositor comments below

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-P1-N-miRFP670-C

    Plasmid

    #159438

    Purpose

    CRISPR knock-in donor construct with fluorescent reporter

    Depositor

    Jianhua Xing

    Article

    Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

    Insert

    miRFP670
    

    Use

    CRISPR

    Tags

    3' linker pair 1, 5' linker pair 1, C t…

    Promoter

    CMV

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-P1-N-EGFP-C

    Plasmid

    #159436

    Purpose

    CRISPR knock-in donor construct with fluorescent reporter

    Depositor

    Jianhua Xing

    Article

    Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    3' linker pair 1, 5' linker pair 1, C t…

    Promoter

    CMV

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-GG

    Plasmid

    #213039

    Purpose

    AAV backbone for Golden Gate assembly of donor templates using BbsI/BpiI (overhangs AGCG and GCAA, respectively)

    Depositor

    Fredrik Sterky

    Article

    De La Cruz et al Bio Protoc. 2024 Nov 5;14(21):e5097. doi

    Type

    Empty backbone

    Use

    AAV; Vector for golden gate assembly

    Tags

    N/A

    Promoter

    N/A

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-P1-N-mCherry-C

    Plasmid

    #159437

    Purpose

    CRISPR knock-in donor construct with fluorescent reporter

    Depositor

    Jianhua Xing

    Article

    Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

    Insert

    mCherry
    

    Use

    CRISPR

    Tags

    3' linker pair 1, 5' linker pair 1, C t…

    Promoter

    CMV

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYJ41-AAVS1-XLone-Mafa

    Plasmid

    #131084

    Purpose

    Donor plasmid of targeted inducible-Mafa knockin

    Depositor

    Xiaojun Lian

    Insert

    MAFA (MAFA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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