We narrowed to 99 results for: donor

Studying the biophysics of Cas9 DNA interaction, the Corn lab has developed a means to enhance CRISPR genome editing through homology directed repair.

Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental procedures.

Learn how to target donor and acceptor fluorophores for FRET experiments.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

Learn what FRET is and how you can use it in your experiments. Find additional FRET resources throughout the post.

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.

The basics of Fluorescence Resonance Energy Transfer (FRET), including tips and parameters to consider when using FRET for your experiments.

CRISPR plasmids for endogenous tagging of your gene of interest.

Find plasmids and resources for FRET experiments to measure protein interactions or conformation changes.

Vectors containing the piggyBac transposon are now available at Addgene. Learn how these unique tools can be used for genome engineering!

Find out more about the different homology directed repair pathways and how to design HDR templates for CRISPR editing.

Find out how you can use new CRISPR tagging plasmids from the Allen Institute for Cell Science to tag your cellular structure of interest.

Find bacterial expression plasmids that can be used for protein purification, protein visualization, study of protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria.

SapTrap improves CRISPR genomic tagging in C. elegans by offering scientists an easy one tube assembly reaction to generate high-throughput targeting vectors.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

Learn how to use Easi-CRISPR to generate mouse knock-in and conditional allele models.

Learn about CRISPR plasmids at Addgene such as an E. coli genome-wide CRISPRi library, GeneWeld vectors, CasX plasmids, and drug inducible CRISPR/Cas9 activation.

Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA.

Scientists are not limited to restriction enzyme cloning. Gateway cloning is a rapid and efficient way to move DNA into multiple vector systems.

Learn about the many fluorescent protein tools enabled by the nanoLuc luciferase.