We narrowed to 4 results for: donor

...This fragment can then be cloned into a Gateway donor plasmid, which contains compatible attP sites via...can clone your gene of interest one time into a donor plasmid or acquire one that already has your gene...Addgene's collection contains thousands of Gateway donor , Gateway entry , and Gateway destination vectors...allowing for rapid cloning of PCR products into donor plasmids without the need for restriction enzyme...

...specific genomic edits by copying from a DNA donor template . The donor template contains the desired sequence...out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration for...cell along with the gRNA(s) and Cas enzyme. The donor DNA contains the desired edit as well as additional...homology arms. Depending on the application, the donor DNA may be a single-stranded oligonucleotide, double-stranded...for large edits or insertions. When designing the donor template, incorporating mutations that prevent further...should alter the PAM site or target sequence in your donor DNA with a silent mutation that does not change ...recombinase or phage derived serine integrases, insert donor DNA at “landing sites” defined by relatively short...

... or small tag insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins that...HDR efficiency . Remember that HDR also requires donor DNA to serve as template for the repair. Once you...you settle on your gRNA, you can design a donor DNA to have the appropriate left and right homology arms...

...mechanisms. One of these methods uses splice site donor and acceptor sequences to reconstitute the two pieces...contains the 5' portion of the gene and a splice donor sequence at its 3' end, while the second vector ...