We narrowed to 81 results for: donor

Studying the biophysics of Cas9 DNA interaction, the Corn lab has developed a means to enhance CRISPR genome editing through homology directed repair.

March 24, 2016, 2:30 p.m.

Learn how to target donor and acceptor fluorophores for FRET experiments.

July 19, 2016, 2:30 p.m.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

Dec. 22, 2022, 2:15 p.m.

Learn what FRET is and how you can use it in your experiments. Find additional FRET resources throughout the post.

June 27, 2017, 1:03 p.m.

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.

Sept. 1, 2020, 1:15 p.m.

The basics of Fluorescence Resonance Energy Transfer (FRET), including tips and parameters to consider when using FRET for your experiments.

Nov. 5, 2014, 3:41 p.m.

Vectors containing the piggyBac transposon are now available at Addgene. Learn how these unique tools can be used for genome engineering!

May 31, 2016, 3:30 p.m.

Find out more about the different homology directed repair pathways and how to design HDR templates for CRISPR editing.

Jan. 26, 2023, 2:15 p.m.

Find out how you can use new CRISPR tagging plasmids from the Allen Institute for Cell Science to tag your cellular structure of interest.

April 6, 2017, 1:02 p.m.

SapTrap improves CRISPR genomic tagging in C. elegans by offering scientists an easy one tube assembly reaction to generate high-throughput targeting vectors.

Oct. 12, 2017, 2:26 p.m.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

March 13, 2014, 4:08 p.m.

Learn how to use Easi-CRISPR to generate mouse knock-in and conditional allele models.

April 5, 2018, 12:42 p.m.

Learn about CRISPR plasmids at Addgene such as an E. coli genome-wide CRISPRi library, GeneWeld vectors, CasX plasmids, and drug inducible CRISPR/Cas9 activation.

Sept. 10, 2019, 12:56 p.m.

Scientists are not limited to restriction enzyme cloning. Gateway cloning is a rapid and efficient way to move DNA into multiple vector systems.

Jan. 12, 2017, 3:30 p.m.

Learn about the many fluorescent protein tools enabled by the nanoLuc luciferase.

Feb. 8, 2018, 12:17 p.m.

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

Dec. 10, 2020, 1 p.m.

Learn about the different ways you can introduce DNA or RNA into cells using methods such as transformation, transduction, conjugation, and transfection.

June 25, 2019, 12:54 p.m.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

June 28, 2018, 3:01 p.m.

Learn how to use Cas9 nickases for genome editing, including homology directed repair.

March 15, 2018, 12:59 p.m.

Mobile-CRISPRi is a set of modular CRISPRi tools that can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration and is easily transferred via conjugation.

April 4, 2019, 12:53 p.m.