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We narrowed to 13 results for: donor

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Showing: 1 - 13 of 13 results

  1. CRISPR Plasmids - Tagging

    Type
    Collection
    ... targeting the genomic site and the donor vector. The PITCh donor plasmid with an EGFP-2A-PuroR cassette... and donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the PITCh donor plasmid...may be used. The HDR (homology directed repair) donor plasmid with homology arms and selection marker....as design and clone in the homology arms for the donor plasmid. The Mendenhall and Myers labs have also... and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor...instead of HDR, which makes it easy to construct a donor vector, because MMEJ utilizes very short (around..., & FBL-specific gRNA pCRIS-PITChv2-FBL - PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL...

  2. Fluorescent Proteins: FRET

    Type
    Collection
    ... , EGFP-ShadowY λ Dex : Donor maximum excitation wavelength (nm), QY D : Donor quantum yield, λ Aem : ... is non-radiatively transferred from an excited donor fluorophore to an acceptor. Because this process...fluorescence microscopy or spectroscopy by exciting the donor and detecting the emission of the acceptor. Alternatively...the fluorophore and emission of a photon) of the donor can be measured using fluorescence lifetime imaging...increased energy transfer through FRET reduces the donor lifetime. FRET is often used to study 1) protein-protein...interactions where each protein is separately fused to a donor or acceptor molecule (also referred to as intermolecular...conformational changes within a protein where the donor and acceptor are both fused to the same protein ...

  3. Bacterial Expression Systems

    Type
    Collection
    ...fluorescence both from the donor and the acceptor fluorophore by only exciting the donor. In BiFC, proteins of...mRuby2-pBAD Clover (donor) mRuby2 (acceptor) FRET Michael Davidson 18856 pGWF1 ECFP (donor) Venus (acceptor...pFLIP38 ECFP (donor) Citrine (acceptor) FRET Wolf Frommer 65617 pFLIP42 mCerulean (donor) Citrine (acceptor...microscopy. FRET relies on the principle that when a donor fluorophore is excited, it can transfer its energy...Application PI 14030 14031 pCyPet-His pYPet-His CyPet (donor) YPet (acceptor) FRET Patrick Daugherty 18084 54856...54856 pBad-mAmetrine1.1 tdTomato-pBAD mAmetrine1.1 (donor) tdTomato (acceptor) FRET/Dual FRET Robert Campbell...Tsien 54553 54723 mTFP1-pBAD mCitrine-pBAD mTFP1 (donor) mCitrine (acceptor) FRET/Dual FRET Robert Campbell...

  4. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...CRISPR-associated transposases (CASTs) for insertion of donor DNA....a guide RNA. The transposase can then integrate donor DNA. Editing by CASTs is not considered "scarless...homology sequences are integrated along with the donor DNA. The two main classes of CASTs are type I and...

  5. CRISPR References and Information

    Type
    Collection
    ...algorithm to automate and optimize gRNA and HDR donor design. The current version supports SpCas9, SpCas9...pAC93 , pAC94 , pAC95 ; dCas9 activator gateway donors: pAC84 , pAC1 , pAC147 , pAC148 , pAC149 ; Gateway...HDR design; Deep sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED...

  7. CRISPR Guide

    Type
    Collection
    ...specific genomic edits by copying from a DNA donor template . The donor template contains the desired sequence...out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration for...cell along with the gRNA(s) and Cas enzyme. The donor DNA contains the desired edit as well as additional...homology arms. Depending on the application, the donor DNA may be a single-stranded oligonucleotide, double-stranded...for large edits or insertions. When designing the donor template, incorporating mutations that prevent further...should alter the PAM site or target sequence in your donor DNA with a silent mutation that does not change ...recombinase or phage derived serine integrases, insert donor DNA at “landing sites” defined by relatively short...

  9. Plan Your Experiment

    Type
    Collection
    ... or small tag insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins that...HDR efficiency . Remember that HDR also requires donor DNA to serve as template for the repair. Once you...you settle on your gRNA, you can design a donor DNA to have the appropriate left and right homology arms...

  10. Distribution to Industry

    Type
    Collection
    ...Mutation Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation...

  11. Promega Plasmid Collection

    Type
    Collection
    ... assays use NanoLuc Luciferase as a BRET energy donor and HaloTag protein, labeled with the NanoBRET 618...

  12. Luciferase Plasmid Collection

    Type
    Collection
    ...NanoBRET : Constructs using NanoLuc® as a BRET energy donor and HaloTag labeled with a fluorophore as the acceptor...

  13. Zhang Lab CRISPR Page

    Type
    Collection
    ...also contains a Kras G12D homology directed repair donor template. #60225 - AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc...
Showing: 1 - 13 of 13 results