Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 16,431 results for: LEA

Clear keywords
Showing: 7981 - 8000 of 16431 results

  4. GFP-DroshaS300E/S302D

    Plasmid
    #62531
    Purpose
    Serine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acid
    Depositor
    Bharat Ramratnam
    Article
    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-
    Insert
    human Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    changed serine 300 to glutamic acid and serine 3…
    Available Since
    March 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pNOC_dfnCas12a-KRAB_crRNA NC

    Plasmid
    #176262
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176262-plasmid-map-sequence-id-346962

    Enlarge

  7. SaCas9 variant click editor (CE1) - pCMV-T7-PCV2-nSaCas9-EcKlenow (MLE194)

    Plasmid
    #217801
    Purpose
    Variant CE1 construct with SaCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSaCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSaCas9(N580A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217801-plasmid-map-sequence-id-432878

    Enlarge

  8. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

    Plasmid
    #122085
    Purpose
    Express M.EcoGII fused to CENPC with destabilization domain - inducible
    Depositor
    Laure Crabbe
    Article
    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5
    Insert
    DD-linker-M.EcoGII-V5-CENPC (CENPC Human, E.coli)
    Use
    Retroviral
    Tags
    V5 tag on Nter of CENPC
    Expression
    Bacterial and Mammalian
    Mutation
    L341F, R930L (see comments below)
    Available Since
    March 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122085-plasmid-map-sequence-id-238127

    Enlarge

  9. CRHR1-DuET

    Plasmid
    #213215
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CRHR1 (CRHR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213215-plasmid-map-sequence-id-430777

    Enlarge

  11. Cherry-HA-KCC2

    Plasmid
    #104077
    Purpose
    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)
    Depositor
    Igor Medyna
    Article
    Kahle et al EMBO Rep. 2014 Jul;15(7):766-74. doi: 10
    Insert
    KCC2 (b isoform) (Slc12a5 Rat)
    Tags
    mCherry-HA tag
    Expression
    Mammalian
    Mutation
    EcoR1 site in rat KCC2 was removed by silent muta…
    Promoter
    UbC
    Available Since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104077-plasmid-map-sequence-id-199357

    Enlarge

  14. pelB-MBP-P66-ss

    Plasmid
    #137045
    Purpose
    E. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66949.1 (p66 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature P66: lacks the P66 signal peptide
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137045-plasmid-map-sequence-id-291951

    Enlarge

  17. pBactin-Nedd1-mOrange2-hCM1

    Plasmid
    #196863
    Purpose
    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-hCM1 (Nedd1 Human, Rat)
    Expression
    Mammalian
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196863-plasmid-map-sequence-id-391832

    Enlarge

  19. cryS96

    Plasmid
    #67519
    Purpose
    αB-crystallin minipeptide fused to ELP (S96).
    Depositor
    Andrew MacKay
    Article
    Wang et al J Control Release. 2014 Oct 10;191:4-14.
    Insert
    mini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Human, Synthetic)
    Tags
    elastin-like polypeptide (ELP) S96
    Expression
    Bacterial
    Mutation
    mini-αB-crystallin contains residues 73–92 of aB-…
    Promoter
    T7
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 7981 - 8000 of 16431 results