Hot Plasmids: Summer 2024
Type
Blog Post
...MagIC-Cryo-EM for enrichment and structure determination
LEA proteins protect samples during cryo-EM grid freezing...: https://doi.org/10.1101/2024.01.21.576499.
LEA proteins protect fragile samples during cryo-EM grid...lab found that adding Late Embryogenesis Abundant (LEA) proteins, small proteins that naturally adsorb to...B) Protein samples protected from AWI by helical LEAs. C-E) cryo-EM single-particle analysis of PRC2 with...under CC-BY-NC-ND 4.0 license.
Protection by the LEAs allowed the authors to determine structures at comparable...nearly ten times less protein. While the addition of LEAs may bias the distribution of particle orientations...-stage imaging. A truncated form of a tardigrade LEA, RvLEAMshort, protected samples and was compatible...