We narrowed to 16,074 results for: LEA

1. Human Lentiviral sgRNA Library - Nuclear

   Pooled Library

   #51047

   Purpose

   Sub-pool of Sabatini/Lander sgRNA library enriched for nuclear targets.

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

2. CHyMErA LbCas12a Nuclease Optimization hgRNA Library

   Pooled Library

   #209734

   Purpose

   CHyMErA Cas12a Nuclease Optimization hgRNA Libraries allows assessment and comparison of the efficacy of different LbCas12a nucleases in the context of the Cas9-Cas12a CHyMErA system.

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Expression

   Mammalian

   Use

   CRISPR and Lentiviral

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

3. CHyMErA AsCas12a Nuclease Optimization hgRNA Library

   Pooled Library

   #209735

   Purpose

   CHyMErA Cas12a Nuclease Optimization hgRNA Libraries allows assessment and comparison of the efficacy of different AsCas12a nucleases in the context of the Cas9-Cas12a CHyMErA system.

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Expression

   Mammalian

   Use

   CRISPR and Lentiviral

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

4. hMEF2A WT

   Plasmid

   #118354

   Purpose

   This plasmid expresses human MEF2A-1 WT.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hMEF2A (MEF2A Human)
   

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Gal4-VP16

   Plasmid

   #71728

   Purpose

   plasmid expressing fusion construct of Gal4 DBD and VP16 AD

   Depositor

   Lea Sistonen

   Article

   Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

   Insert

   Gal4 DBD(1-147) (GAL4 Budding Yeast)
   

   Tags

   VP16 AD (413-490)

   Expression

   Mammalian

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. hHSF1 RNAi resistant

   Plasmid

   #118347

   Purpose

   This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).

   Depositor

   Lea Sistonen

   Article

   Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

   Insert

   hHSF1 (HSF1 Human)
   

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   RNAi resistance mutations (does not change amino …

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-hHSF1 WT

   Plasmid

   #71724

   Purpose

   plasmid expressing myc-his tag hHSF1 WT

   Depositor

   Lea Sistonen

   Article

   Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

   Insert

   HSF1 (HSF1 Human)
   

   Tags

   MYC 6xHIS

   Expression

   Mammalian

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSUPER-scramble

   Plasmid

   #118349

   Purpose

   This plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.

   Depositor

   Lea Sistonen

   Article

   Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

   Insert

   scrambled shRNA
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. hSUMO1 GFP

   Plasmid

   #118360

   Purpose

   This plasmid expresses the full length human SUMO1.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hSUMO1 (SUMO1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. hGATA-1 WT

    Plasmid

    #118352

    Purpose

    This plasmid expresses human GATA-1 WT.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hGATA-1 (GATA1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. hHSF1 K80Q

    Plasmid

    #118351

    Purpose

    Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

    Depositor

    Lea Sistonen

    Article

    Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

    Insert

    hHSF1 (HSF1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K80Q

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. hHSF1 K80R

    Plasmid

    #118350

    Purpose

    Plasmid expresses hHSF1 with K80 in DBD mutated to R.

    Depositor

    Lea Sistonen

    Article

    Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

    Insert

    hHSF1 (HSF1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K80R

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mNeonGreen-Smad3

    Plasmid

    #172098

    Purpose

    Expresses fluorescently tagged human Smad3 in mammalian cells.

    Depositor

    Lea Goentoro

    Article

    Frick et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    Smad 3 (Smad3 Mouse)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    C49S mutation in mCerulean

    Promoter

    CMV

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSUPER-hHSF1 shRNA

    Plasmid

    #118345

    Purpose

    This plasmid expresses shRNA against hHSF1.

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    hHSF1 shRNA (HSF1 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. hHSF1 S303/7A

    Plasmid

    #118365

    Purpose

    This plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    S303A and S307A

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. hSUMO1-HIS

    Plasmid

    #118361

    Purpose

    This plasmid expresses the full length human SUMO1.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hSUMO1 (SUMO1 Human)
    

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Please see Depositor Comments

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. hHSF4b WT

    Plasmid

    #118356

    Purpose

    This plasmid expresses human HSF4b WT.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-HSF1Δ∼PRD

    Plasmid

    #71725

    Purpose

    plasmid expressing mic-his tag phosphorylation-deficient hHSF1

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    phosphorylation-deficient HSF1 (HSF1 Human)
    

    Tags

    mac 6xHIS

    Expression

    Mammalian

    Mutation

    15 phosphorylation sites in RD mutated to Alanine

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. hGATA-1 K137R

    Plasmid

    #118353

    Purpose

    This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hGATA-1 (GATA1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K137R

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. hHSF1 K298R

    Plasmid

    #118362

    Purpose

    This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K298R

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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