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FSW mKeima

Plasmid

#246726

Purpose

Neuron-specific expression of insert with a mKeima tag on its N-terminus after cloning

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Tags

mKeima

Expression

Mammalian

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_CTRL_ER-dAPEX-BFP2

Plasmid

#236733

Purpose

Dual sgRNA targeting CTRL expressing dAPEX-BFP targeting to ER

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

Negative CTRL

Use

CRISPR and Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd1/2 gRNA

Plasmid

#246562

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd1/2 gRNA (conserved region)

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd1/2 gRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBABE-Puro-BirA

Plasmid

#246557

Purpose

Retroviral expression vector for secreted E. coli BirA, C-terminal FLAG tag

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

BirA

Use

Retroviral

Tags

FLAG

Promoter

MMLV LTR

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti mKeima

Plasmid

#246746

Purpose

Mammalian and lentiviral expression of insert with a mKeima tag on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Tags

mKeima

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pQGG001

Plasmid

#231313

Purpose

Vector part for BEVA; BEVA2.0 Position 4 I-SceI recognition/cut site, SpR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 4 I-SceI recognition/cut site, SpR.

Expression

Bacterial

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-C OR (T AND V)-CAAX

Plasmid

#243791

Purpose

Expresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from membranes in response to HRV-3C OR (TEV AND TVMV) treatment. Construct contains a CAAX tag for intracellular membrane tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Use

Lentiviral

Tags

CAAX Tag

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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GST-Thrombin-Munc13-1 MUN domain (aa859-1516 delta1408-1452 EF)

Plasmid

#243818

Purpose

Munc13-1 protein purification

Depositor

Jose Rizo-Rey

Article

Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

Insert

Munc13-1 (Unc13a Rat)

Tags

GST

Expression

Bacterial

Mutation

1408-1452 --> EF

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-FGD3

Plasmid

#242832

Purpose

Encodes human FGD3 and N-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

FGD3 (FGD3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-ARHGEF6

Plasmid

#242830

Purpose

Encodes human ARHGEF6 and N-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

ARHGEF6 (ARHGEF6 Human)

Tags

EGFP

Expression

Mammalian

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA_H1-5

Plasmid

#223820

Purpose

Bacterial expression of SynPspA H1-5 with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Mutation

truncation of helix 0

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA

Plasmid

#223818

Purpose

Bacterial expression of SynPspA with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pX330 - LRR1 Terminal CRISPR

Plasmid

#221705

Purpose

Cas9 targeting plasmid with gRNA specific for LRR1 N-terminus

Depositor

Aga Gambus

Article

Jones et al iScience. 2024 Jun 12;27(7):110260. doi:

Insert

TGTAGCTTCATCTCGCCCAA

Use

CRISPR

Promoter

Chicken Beta-actin

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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LSAM domain expression plasmid

Plasmid

#240215

Purpose

Plasmid for the recombinant expression of the LSAM domain of human legumain in E. coli.

Depositor

Elfriede Dall

Article

Dahms et al STAR Protocols, 6, 3, 103991, 19 Septemb

Insert

LSAM (LGMN Human)

Tags

PC1pro

Expression

Bacterial

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1369

Plasmid

#239828

Purpose

WT OrufIscB mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-NanA-CSS-ET64

Plasmid

#234666

Purpose

Expression an ELP-tagged fusion enzyme of NanA and CSS

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

sialic acid aldolase
CMP-sialic acid synthetase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-ManC-NahK-ET64

Plasmid

#234664

Purpose

Expression an ELP-tagged fusion enzyme of PfManC and NahK

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

GDP-Man pyrophosphorylase
N-acetylhexosamine 1-kinase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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Gai-mTagBFP-3BEY-P2A-Gid-4LTB-GFP

Plasmid

#240137

Purpose

Mammalian plasmid expression both components of synDrops separated using P2A

Depositor

Liam Holt

Article

Shu et al PRX Life. 2024 Sep;2(3):033001. doi: 10.

Inserts

Gai-mTagBFP-3BEY
Gid-4LTB-GFP

Expression

Bacterial and Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1376

Plasmid

#239829

Purpose

WT OrufIscB omegaRNA mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB omegaRNA scaffold

Expression

Mammalian

Promoter

human U6

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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YEE_GFP

Plasmid

#228184

Purpose

Yeast episomal expression vector with type '234' GFP drop out site. For use in assembling protein expression cassettes via golden gate assembly.

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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