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Addgene
Showing: 1 - 20 of 42 results

  1. PUF Assembly Kit

    Plasmid Kit
    #1000000051
    Purpose
    Used to assemble custom-made RNA-binding proteins for recognition of 8-base long targets within RNA. The kit is based on the Pumilio and FBF (PUF) homology domain of the full-length human Pumilio 1.
    Depositor
    Huimin Zhao
    Availability
    Academic Institutions and Nonprofits only

  2. FusX TALEN assembly system

    Plasmid Kit
    #1000000063
    Purpose
    Golden Gate assembly of 15-16 RVD TALEN inserts in a single cloning step; designed to be used with high throughput robotics
    Depositor
    Stephen Ekker
    Availability
    Academic Institutions and Nonprofits only

  3. STAR TALE Assembly Kit

    Plasmid Kit
    #1000000117
    Purpose
    These plasmids use a TALE assembly reaction (STAR) method to construct multiple TALEs in a fast and simple manner.
    Depositor
    Steve Pollard
    Availability
    Academic Institutions and Nonprofits only

  4. Mobius Assembly Vector Toolkit

    Plasmid Kit
    #1000000134
    Purpose
    Mobius Assembly: Uses a two-level approach for transcriptional unit (TU) and multi-TU augmentation. Each level is comprised of four Acceptor Vectors. Compatible with Golden Gate parts
    Depositor
    Naomi Nakayama
    Availability
    Academic Institutions and Nonprofits only

  5. Start-Stop Assembly Toolkit

    Plasmid Kit
    #1000000152
    Purpose
    Start-Stop Assembly is a multi-part, modular DNA assembly method which is functionally scarless, suitable for combinatorial assembly, and ideal for metabolic engineering in diverse organisms.
    Depositor
    John Heap
    Availability
    Academic Institutions and Nonprofits only

  6. MetClo DNA Assembly Kit

    Plasmid Kit
    #1000000153
    Purpose
    MetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.
    Depositor
    Christopher A. O'Callaghan
    Availability
    Academic Institutions and Nonprofits only

  7. Diatom uLoop Assembly Kit

    Plasmid Kit
    #1000000238
    Purpose
    The collection contains a selection of diatom promoters and terminators from the organism Phaeodactylum tricornutum on vectors compatible with the uLoop cloning system.
    Depositor
    Christopher Dupont
    Availability
    Academic Institutions and Nonprofits only

  8. LIC TAL Effector Assembly Kit

    Plasmid Kit
    #1000000023
    Purpose
    LIC (ligation-independent cloning) TALE repeat unit library of 2-mer fragments for assembly in an expression ready TALEN construct in 2 hierarchical assembly steps. Semi‐automated TALE generation
    Depositor
    Veit Hornung
    Availability
    Academic Institutions and Nonprofits only

  9. Shen Lab DdCBE assembly kit

    Plasmid Kit
    #1000000212
    Purpose
    The DddA-derived cytosine base editor (DdCBE) assembly kit is a collection of 192 modules harboring repeat variable di-residue (RVD) arrays for any desired target.
    Depositor
    Bin Shen
    Availability
    Academic Institutions and Nonprofits only

  10. Joung Lab REAL Assembly TALEN Kit

    Plasmid Kit
    #1000000017
    Purpose
    Used to assemble any combination of TALE repeats into arrays of any desired length using the REAL, REAL-Fast or FLASH assembly methods.
    Depositor
    Keith Joung
    Availability
    Academic Institutions and Nonprofits only

  12. Multiplex CRISPR/Cas9 Assembly System Kit

    Plasmid Kit
    #1000000055
    Purpose
    Golden Gate cloning assembly for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either Cas9 nuclease or Cas9 D10A nickase; see kit #1000000062 for FokI Accessory Pack
    Depositor
    Takashi Yamamoto
    Availability
    Academic Institutions and Nonprofits only

  16. Musunuru-Cowan TALEN Kit

    Plasmid Kit
    #1000000034
    Purpose
    Used to efficiently assemble TALEN constructs with custom repeat arrays, each containing 15 repeats. Contains pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly.
    Depositor
    Kiran Musunuru
    Availability
    Academic Institutions and Nonprofits only

  17. dRMCE Plasmids

    Plasmid Kit
    #1000000015
    Purpose
    Takes advantage of wild-type loxP and FRT sites present in these conditional alleles in mouse embryonic stem cells to introduce tags, reporters, and mutant coding regions into an endogenous loci
    Depositor
    Rolf Zeller
    Availability
    Academic Institutions and Nonprofits only

  18. biGBac Kit

    Plasmid Kit
    #1000000088
    Purpose
    Generation of baculoviral transfer vectors coding up to 25 protein complex subunits in two Gibson assembly steps. Multisubunit protein complexes can be generated in baculovirus-infected insect cells.
    Depositor
    Jan-Michael Peters
    Availability
    Academic Institutions and Nonprofits only

  19. AdenoBuilder toolkit

    Plasmid Kit
    #1000000176
    Purpose
    Plasmids containing modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction.
    Depositor
    Fred Bunz
    Availability
    Academic Institutions and Nonprofits only

  20. Zinc Finger Consortium Expression Vector Kit v1.0

    Plasmid Kit
    #1000000010
    Purpose
    Contains plasmids and strains for the bacterial two-hybrid (B2H) system used to screen zinc finger arrays for activity. Also called the Modular Assembly Accessory Reagents Kit.
    Depositor
    Keith Joung
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 42 results