We narrowed to 14,953 results for: pET

1. pPB-CAG-ECC-Biosensor

   Plasmid

   #140832

   Purpose

   Encoding the CAG promoter based eccDNA biosensor

   Depositor

   Yonglun Luo

   Article

   Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

   Insert

   ECC-Biosensor
   

   Use

   Piggybac (pb) transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-tdTEVDB_C-15A/G4C

   Plasmid

   #177205

   Purpose

   Permuted td intron with evolved initiation sequence for the expression of circular mRNA

   Depositor

   Stephen Fried

   Article

   Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175

   Insert

   Permuted GFP with evolved initiation sequence for circular mRNA
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Permuted sfGFP after residue 52 & Changed Cyt…

   Promoter

   araBAD

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. MMLV-RT(dRH) for Split-PE (pEH50)

   Plasmid

   #190106

   Purpose

   Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   MMLV-RT(delta RNAseH)
   

   Use

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   mutations from RT in PE2 and truncation of RNAse …

   Promoter

   CMV

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSynapsin1-GCaMP6s-P2A-mKate2

   Plasmid

   #112006

   Purpose

   For co-expression of GCaMP6s with mKate2

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Has Service

   AAV9

   Insert

   GCaMP6s-P2A-mKate2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-FLAG-SBP-STAG2 (WT)

   Plasmid

   #73963

   Purpose

   STAG2 cDNA (CCDS43990) wild type

   Depositor

   Todd Waldman

   Article

   Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

   Insert

   STAG2 (STAG2 Human)
   

   Use

   Tags

   FLAG-SBP

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pR26 CAG/BFP Dest

   Plasmid

   #74282

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSMART-sgRNA (Sp)

   Plasmid

   #80427

   Purpose

   U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

   Depositor

   Melissa Little, Sara Howden

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMVHA E2F2

   Plasmid

   #24226

   Purpose

   Mammalian expression of human E2F2 with HA tag

   Depositor

   Kristian Helin

   Article

   Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

   Insert

   E2F2 (E2F2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. His-AgdNK

   Plasmid

   #203226

   Purpose

   For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
   

   Use

   Tags

   N-terminal TEV cleavable 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSNRW-Z5-Nluc

    Plasmid

    #174487

    Purpose

    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

    Depositor

    Richard C. Willson

    Article

    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

    Insert

    Z5-Nluc
    

    Use

    Tags

    6x Histidine tag (N terminal on insert)

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-mApple-6xHp

    Plasmid

    #217376

    Purpose

    Lipid droplet localization of 6xHp of Spastin fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    mApple-6x Hp NC
    

    Use

    Lentiviral

    Tags

    mApple

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cas9-2A-Cre

    Plasmid

    #68468

    Purpose

    co-expressing Cas9 and Cre recombinase

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    Cas9 and Cre recombinase
    

    Use

    CRISPR

    Tags

    P2A-Cre-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.U6gLacZ.hSyn.flex.H2B.RFP

    Plasmid

    #170373

    Purpose

    Negative control guide, Cre-inducible plasmid expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    Cre inducible H2B RFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-TRE-ECC-Biosensor

    Plasmid

    #140833

    Purpose

    Encoding the TRE promoter based eccDNA biosensor

    Depositor

    Yonglun Luo

    Article

    Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

    Insert

    TRE-ECC Biosensor
    

    Use

    Piggybac (pb) transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    inverse

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCIFR-3

    Plasmid

    #233295

    Purpose

    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    msfGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    p14G

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCX-SpiCee-NLS

    Plasmid

    #140900

    Purpose

    mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee-NLS
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-PCNA-IRES-puro2b

    Plasmid

    #26461

    Depositor

    Daniel Gerlich

    Article

    Held et al Nat Methods. 2010 Sep . 7(9):747-54.

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    pEGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF1-alpha-IIb

    Plasmid

    #27288

    Depositor

    Timothy Springer

    Article

    Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

    Insert

    integrin alpha IIb (ITGA2B Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. FAB(ER-LD)

    Plasmid

    #214424

    Purpose

    Detection of ER-lipid droplet contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    FRB-V5
    

    Use

    Tags

    CFAST-Halo and RspAN-FRB-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-FLAG-ATBF1

    Plasmid

    #40927

    Depositor

    Jin-Tang Dong

    Article

    Dong et al J Biol Chem. 2010 Oct 22;285(43):32801-9

    Insert

    ATBF1 (ZFHX3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    CMV

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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