We narrowed to 15,188 results for: pET

1. pVRb20_992

   Plasmid

   #49714

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   sfgfp
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Pecf20_992

   Available Since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MSP977

   Plasmid

   #65774

   Purpose

   Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135E mutation in Cas9

   Promoter

   CMV & T7

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MMLV-RT for Split-PE (pEH45)

   Plasmid

   #190105

   Purpose

   Expresses MMLV-RT (full-length, from PE2) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   MMLV-RT
   

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   RT pentamutant from PE2 (D200N, T306K, W313F, T33…

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAM232

   Plasmid

   #226654

   Purpose

   pET24b-p97-FLAG

   Depositor

   Andreas Martin

   Article

   Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

   Insert

   p97 (VCP Human)
   

   Tags

   FLAG

   Expression

   Bacterial

   Available Since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NFAST(low)-mApple-6xHp

   Plasmid

   #214412

   Purpose

   Lipid droplet localization of low-affinity NFAST fused to mApple-6xHp

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   RspA-N
   

   Tags

   mApple-6xHp

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pVRa20_992

   Plasmid

   #49673

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF20_992
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available Since

   Jan. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEYFP-Pds1(434)

   Plasmid

   #39847

   Depositor

   Jonathon Pines

   Article

   Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

   Insert

   Pds1 (PTTG1 Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHS1184

   Plasmid

   #205965

   Purpose

   NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   DinG HNH
   

   Tags

   14xHis-TwinStrep-bdSUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TALEN1257

   Plasmid

   #32280

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   TAL array targeting hey2 (hey2 Zebrafish)
   

   Use

   T7

   Tags

   3X FLAG and WT FOKI

   Expression

   Mammalian

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHis-zfEpoa

    Plasmid

    #65611

    Purpose

    Express soluble His-tagged zebrafish erythropoietin in E. coli

    Depositor

    Petr Bartunek

    Article

    Stachura et al Blood. 2009 Jul 9;114(2):279-89. doi: 10

    Insert

    erythropoietin (epoa Zebrafish)
    

    Tags

    (His)6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt

    Plasmid

    #194519

    Purpose

    Dual fluorescence reporter for studying protein degradation

    Depositor

    Tommer Ravid

    Article

    Mashahreh et al Nat Commun. 2022 Dec 8;13(1):7588. doi:

    Insert

    TIF4631 leader sequence
    

    Expression

    Yeast

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOPS0263

    Plasmid

    #133228

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with PsNifH (pOGG043), gusA (pOGG083) and T-pharma (pOGG003)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOPS0262

    Plasmid

    #133227

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with PsNifH (pOGG043), celB (pOGG050) and T-pharma (pOGG003)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. G1333 DddAtox-N–dSpCas9

    Plasmid

    #157833

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAM4937 (pCV0049)

    Plasmid

    #120082

    Purpose

    Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 2 (NS2).

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS2, CV-aph1, CV-ccdB-SwaI
    

    Use

    Other

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-hSynapsin1-axon-GCaMP6m

    Plasmid

    #111261

    Purpose

    For enriched expression of GCaMP6m in axons

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV9

    Insert

    axon-GCaMP6m
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Promoter

    hSynapsin1

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pssAAV_Seq17_CMV_bglobin_3NLS_Venus_WPRE_polyA

    Plasmid

    #220224

    Purpose

    AAV-STARR-seq validation control vector with Venus

    Depositor

    Simon Rumpel

    Article

    Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVRc20_992

    Plasmid

    #49739

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    AS20_992
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pLux

    Available Since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC57-Amp_CRISPIE donor B3

    Plasmid

    #172844

    Purpose

    CRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-0) encoding mTurquoise2
    

    Use

    CRISPR

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHL 1075

    Plasmid

    #53004

    Purpose

    pCon:fhlA::mCherry, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    fhlA
    sodB
    

    Use

    Synthetic Biology

    Tags

    GFP and mCherry

    Expression

    Bacterial

    Mutation

    contains −309 to +96 bp relative to start codon a…

    Promoter

    pCon and pLlacO-1

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only