167,795 results

1. FLAG-HA-pcDNA3.1-

   Plasmid

   #52535

   Purpose

   enables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteins

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   FLAG-HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ki67-mNeonGreen-IRESpuro2

   Plasmid

   #183737

   Purpose

   Expresses Ki67-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 (MKI67 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. DRD1-Tango

   Plasmid

   #66268

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   DRD1 (DRD1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX304-IRES-GFP

   Plasmid

   #176288

   Purpose

   pLX304 backbone with an IRES-GFP cassette subcloned into the NheI-AgeI sites

   Depositor

   William Kaelin

   Article

   Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. XLone-SOX18

   Plasmid

   #163822

   Purpose

   expresses human SOX18 upon doxycycline treatment

   Depositor

   Xiaojun Lian

   Article

   Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

   Insert

   SOX18 (SOX18 Human)
   

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Integrin-Alpha2-N-18

   Plasmid

   #58192

   Purpose

   Localization: Integrin/Focal Adhesions, Excitation: N/A, Emission: N/A

   Depositor

   Michael Davidson

   Insert

   Integrin-Alpha2 (ITGA2 Human)
   

   Tags

   SNAP tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pUFlip-floxed2A-mRFP-; HE1A:BFP -2

   Plasmid

   #180009

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-mRFP; HE1A: BFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223 SARS-CoV-2 N

   Plasmid

   #149330

   Purpose

   Gateway-compatible Entry vector, with insert of the nucleocapsid (N) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   N (N SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. NRCoC

   Plasmid

   #74300

   Purpose

   Mammalian expression of full-length treacle fused to GFP

   Depositor

   Rita Shiang

   Article

   Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

   Insert

   treacle ribosome biogenesis factor 1 (Tcof1 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMV_AA088

    Plasmid

    #216104

    Purpose

    Knockout, EF1a-driven Cas9 (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-GGamma10-GFP2

    Plasmid

    #166779

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma10-GFP2 (GNG10 Human, Synthetic)
    

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMito-iRFP670

    Plasmid

    #45462

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-AtC60αβC20NtL

    Plasmid

    #160876

    Purpose

    Expression of N. tabacum Rubisco large subunit (rbcL) and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Expression

    Bacterial

    Promoter

    rbcL: T7, chaperones: T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pANAP

    Plasmid

    #48696

    Purpose

    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

    Depositor

    Peter Schultz

    Article

    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

    Inserts

    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    

    Expression

    Mammalian

    Mutation

    mutant E.coli leucyl synthetase specific for Anap…

    Promoter

    CMV and human H1 promoter

    Available Since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Dendra2-H2B-6

    Plasmid

    #57724

    Purpose

    Localization: Nucleus Histones, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Insert

    H2B (H2BC11 Human)
    

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pV132-vCas9

    Plasmid

    #203223

    Purpose

    Mammalian expression of vCas9

    Depositor

    Phil Sharp, Robert Langer, Vikash Chauhan

    Article

    Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

    Insert

    vCas9
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Mutation

    S55R R976A K1003A T1314R

    Promoter

    CBh

    Available Since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Human SAM library in lenti sgRNA(MS2)_puro backbone

    Pooled Library

    #1000000074

    Purpose

    Genome-wide CRISPR gRNA pooled library for activation of mouse genes

    Depositor

    Feng Zhang

    Available Since

    March 17, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-Beta3

    Plasmid

    #140988

    Purpose

    Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GBeta3 (GNB3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDF-NtXNtS1R1R2B2

    Plasmid

    #160882

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcS-S1, rbcX, raf1, raf2, bsd2
    

    Expression

    Bacterial

    Promoter

    rbcS-S1: T7, chaperones: T7

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBPp65ADZpUw

    Plasmid

    #26234

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    p65AD-Zip (RELA Human)
    

    Expression

    Insect

    Available Since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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