180,111 results
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Plasmid#140027PurposeTunable and temporal expression control of nuclear eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEnhanced Green Fluorescent Protein
UseSynthetic BiologyExpressionMammalianPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Chromoprotein kit 1.0
Plasmid kit#1000000174PurposeExpression of visible chromoproteins in E. coli.DepositorApplicationFor the Classroom, Gene Expression and LabelingVector typeBacterial ExpressionAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-dLight1.2 (AAV1)
Viral prep#111068-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSyn-dLight1.2 (#111068). In addition to the viral particles, you will also receive purified pAAV-hSyn-dLight1.2 plasmid DNA. Synapsin-driven expression of dLight1.2 dopamine sensor. This sensor has a slightly increased dynamic range and is better for 2P imaging in vivo. These AAV preparations are suitable purity for injection into animals.DepositorAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-Sfp
Plasmid#159617PurposeExpression plasmid for Sfp phosphopantetheinyl transferaseDepositorInsertSfp
Tags6HISExpressionBacterialAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
Plasmid#159816PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Ef1a-Coff/Fon-GCaMP6M (AAV8)
Viral prep#137121-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-GCaMP6M (#137121). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-GCaMP6M plasmid DNA. EF1a-driven expression of GCaMP6m in the presence of Flp recombinase (inhibited in the presence of Cre recombinase). These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
JD553
Plasmid#160392PurposeWheat GRF4-GIF1 chimera in pDONR-zeoDepositorInsertwheat GRF4-GIF1 chimera
UseEntry clonAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR
Plasmid#154003PurposeVector expressed CasRx and sgRNA_LACZ for AAV packageDepositorInsertCasRx
UseAAV and CRISPRPromoterEFSAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-Syn-PSD95.FingR-eGFP-CCR5TC
Plasmid#125693PurposeGlobal labeling of excitatory synapses (green fluorescence)DepositorInsertPSD95.FingR-eGFP-CCR5TC
UseAAVExpressionMammalianPromoterSynapsinAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
tet_pLKO.1_puro_shNRF2 #1
Plasmid#136584PurposeExpresses an inducible short hairpin targeting human NRF2 sequenceDepositorAvailable sinceJune 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX165-HiFi Cas9
Plasmid#140563PurposeExpresses HiFi Cas9 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
MutationR691AAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223 SARS-CoV-2 ORF7B
Plasmid#141277PurposeGateway-compatible Entry vector, with insert of ORF7B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCEP4-myc-HLA-A2
Plasmid#135504PurposeMammalian expression of myc-tagged HLA-A*02:01DepositorInsertHLA-A*02:01 (HLA-A Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
NW50
Plasmid#133445PurposeCovalently circularized nanodisc NW50 for studying membrane proteins and viral entry.DepositorInsertNW50
ExpressionBacterialAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-aBEST
Plasmid#131464PurposeExpression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsStreptomyces codon optimized spCas9n (D10A)
streptomyces codon optimized tadA
UseCRISPR and Synthetic BiologyPromotertipAAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-Sport6-CD63-pHuji
Plasmid#130902PurposeExpression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD63-pHuji (CD63 Human, synthetic)
TagspHujiExpressionMammalianMutationpHuji inserted between Gln-36 and Leu-37 in extra…PromoterCMVAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HR180-LGR5-iCT
Plasmid#129094PurposeKI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Twinkle-APEX2-V5
Plasmid#129705PurposeAPEX2 fused to the mitochondrial nucleoid protein TwinkleDepositorUseLentiviralExpressionMammalianPromoterCMV promoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
amajLime chromoprotein
Plasmid#117843PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coliDepositorInsertpromoter, RBS, amajLime
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT372
Plasmid#125613PurposeExpresses evoCDA1-BE4max-NG in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertevoCDA1-BE4max-NG
ExpressionMammalianMutationF23S A123V I195FAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by