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(Plasmid #20210)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 20210 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5400
  • Modifications to backbone
    The expression plasmid pMC was constructed by inserting a synthetic polylinker (5′-AGC TTG CGG CCG CTA GCC TCG AGG GAT CCT CTA GAA TGC A-3′) into a pcDNA3 vector (Invitrogen) followed by insertion of the chloramphenicol acetyl transferase (CAT) coding sequence. The CAT insert was generated by PCR amplification using pCAT as a template.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    TNFa 3' UTR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    TNF (a.k.a. DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CAT-F (5'-GTTTGTGATGGCTTCCATGTC-3')
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The 3′UTR was generated by PCR amplification from a liver cDNA library (Invitrogen). and the expression plasmids pMC-TNFα was constructed by inserting the 3′UTR of TNFα downstream of CAT in the pMC construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMC-TNFalpha was a gift from Martin Holcik (Addgene plasmid # 20210 ; ; RRID:Addgene_20210)
  • For your References section:

    hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of cIAP1 mRNA. Zhao TT, Graber TE, Jordan LE, Cloutier M, Lewis SM, Goulet I, Cote J, Holcik M. Cell Death Differ. 2009 Feb . 16(2):244-52. 10.1038/cdd.2008.146 PubMed 18846111