167,795 results

1. Yes-EGFP

   Plasmid

   #110497

   Purpose

   Fluorescent Yes

   Depositor

   Bernardo Mainou

   Insert

   Yes (YES1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-hPPP3CB-FLAG

   Plasmid

   #179135

   Purpose

   Expression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

   Insert

   protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCasAb-apr

   Plasmid

   #121998

   Purpose

   Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiTet-LDLR-mCherry BlastR

   Plasmid

   #186739

   Purpose

   Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   LDLR (LDLR Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

   Plasmid

   #213118

   Purpose

   Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   PKAsub-mCherry-MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mPA-GFP-CD151-7

   Plasmid

   #57125

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   CD151 (CD151 Human)
   

   Tags

   mPA-GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pBAD-HisD-mNG-Ec-Kbp-D9N

   Plasmid

   #178011

   Purpose

   Bacterial expression of green fluorescent potassium indicator mNG-Ec-Kbp-D9N (KRaION1 mutant D9N)

   Depositor

   Edward Boyden

   Article

   Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

   Insert

   mNG-Ec-Kbp-D9N
   

   Expression

   Bacterial

   Promoter

   araB

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1a-MinE-IRES-Blast

   Plasmid

   #213115

   Purpose

   Expression of untagged MinE in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   MinE (minE )
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJAT7

   Plasmid

   #204292

   Purpose

   For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-DsRed
   

   Use

   CRISPR

   Promoter

   3XP3

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Myosin-IIA-GFP

    Plasmid

    #38297

    Depositor

    Matthew Krummel

    Article

    Jacobelli et al J Immunol. 2009 Feb 15;182(4):2041-50.

    Insert

    Myosin II A (Myh9 Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

    Plasmid

    #170378

    Purpose

    Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

    Depositor

    Jeremy Day

    Article

    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

    Insert

    A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    human Synapsin promoter

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Human Metabolic Gene CRISPRa sgRNA library

    Pooled Library

    #187080

    Purpose

    Lentiviral CRISPRa library targeting human metabolic genes for activation of gene expression

    Depositor

    Kivanc Birsoy

    Article

    Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

13. pAAV.TBG.PI.Null.bGH

    Plasmid

    #105536

    Purpose

    empty AAV vector with TBG promoter

    Depositor

    James M. Wilson

    Has Service

    AAV8

    Insert

    None
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TBG

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDisplay-GRAB-rDA2m-IRES-EGFP-CAAX

    Plasmid

    #208694

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2m and a membrane-localized EGFP in mammalian cells

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA2m
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

    Plasmid

    #79671

    Purpose

    Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

    Depositor

    Scott Sternson

    Article

    Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMaCTag-P11

    Plasmid

    #120022

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-i

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mScarlet-i
    

    Use

    Pcr template

    Tags

    mScarlet-i

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRosa26-EF1a-hCas9IRESneo

    Plasmid

    #67987

    Purpose

    Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-hCas9IRESneopA
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSC-NLS-RFP

    Plasmid

    #221813

    Purpose

    Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear localization sequence (NLS)

    Depositor

    Baojin Ding

    Article

    Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

    Insert

    NLS-mCherry
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

    Plasmid

    #214102

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE-Plus
    

    Expression

    Mammalian

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. T-STOP sgRNA-1

    Plasmid

    #83346

    Purpose

    The sgRNA at the stop codon of human Brachyury(T) locus

    Depositor

    James Thomson

    Article

    Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

    Insert

    T-STOP sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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