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pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf9c-IRES-Puro

Plasmid

#141393

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf9c

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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pmiRFP720-N1

Plasmid

#136560

Purpose

A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720

Depositor

Vladislav Verkhusha

Publication

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

miRFP720

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pSRE SSG

Plasmid

#127692

Purpose

Plasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.

Depositor

Justin English, Bryan Roth

Publication

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

Sindbis Structural Genome

Expression

Mammalian

Available since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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SpyDock

Plasmid

#124618

Purpose

Expresses SpyDock to bind SpyTag non-covalently for affinity purification

Depositor

Mark Howarth

Publication

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyDock

Tags

His6

Expression

Bacterial

Mutation

E77A mutation prevents isopeptide bond formation,…

Promoter

T7

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-Voltron

Plasmid

#119033

Purpose

Express Voltron in mammalian cells

Depositor

Eric Schreiter

Publication

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Voltron

Expression

Mammalian

Promoter

CAG

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PX458-AAVS1

Plasmid

#113194

Purpose

Encodes gRNA for human AAVS1

Depositor

Adam Karpf

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AAVS1 (PPP1R12C, AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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NES-EGFP-cPHx3

Plasmid

#116855

Purpose

PI(3,4)P2 biosensor

Depositor

Gerry Hammond

Publication

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PLEKHA1 (PLEKHA1 Human)

Tags

EGFP and X.leavis map2k1.L(32-44)

Expression

Mammalian

Mutation

amino acids 169-329 (tandem trimer)

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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PresentER-SIINFEKL (GFP)

Plasmid

#102944

Purpose

PresentER minigene encoding H2-Kb ligand SIINFEKL (GFP)

Depositor

David Scheinberg

Publication

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Insert

SIINFEKL (OVAL Chicken, Synthetic)

Use

Retroviral

Expression

Mammalian

Mutation

OVA257-264

Promoter

LTR

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCAG cyto-RCaMP1h

Plasmid

#105014

Purpose

cytosolic calcium sensor

Depositor

Franck Polleux

Publication

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RCaMP1h

Expression

Mammalian

Promoter

pCAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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MTS-mCherry-GFP1-10-Hyg-N1

Plasmid

#91957

Purpose

Expresses mCherry-GFP1-10 in mammalian cell mitochondria

Depositor

Rong Li

Publication

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MTS-mCherry-GFP1-10

Expression

Mammalian

Promoter

CMV

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pScarlessHD-DsRed

Plasmid

#64703

Purpose

Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Kate O'Connor-Giles

Insert

piggyBac-3xP3-DsRed

Promoter

3xP3

Available since

Dec. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pScarlessHD-DsRed

Plasmid

#64703

Purpose

Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Kate O'Connor-Giles

Insert

piggyBac-3xP3-DsRed

Promoter

3xP3

Available since

Dec. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pVQ CMV NanoV1-2a-EGFP ferritin

Plasmid

#79649

Purpose

Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein

Depositor

Jeffrey Friedman

Publication

Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

Inserts

transient receptor potential vanillin 1 (Trpv1 Rat)
camelid anti-GFP nanobody
Enhanced green fluorescent protein
mouse ferritin light chain (Ftl1 Mouse)
mouse ferritin heavy chain (Fth1 Mouse)

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pVQ CMV NanoV1-2a-EGFP ferritin

Plasmid

#79649

Purpose

Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein

Depositor

Jeffrey Friedman

Publication

Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

Inserts

transient receptor potential vanillin 1 (Trpv1 Rat)
camelid anti-GFP nanobody
Enhanced green fluorescent protein
mouse ferritin light chain (Ftl1 Mouse)
mouse ferritin heavy chain (Fth1 Mouse)

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGWB501

Plasmid

#74843

Purpose

Gateway cloning compatible binary vector for simple cloning of gene.

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGWB501

Plasmid

#74843

Purpose

Gateway cloning compatible binary vector for simple cloning of gene.

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Human Brunello kinome pooled library, guides 1-8 in lentiGuide-Puro backbone

Pooled library

#1000000082

Purpose

CRISPR gRNA knockout pooled library for targeting the human kinome

Depositor

John Doench, David Root

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
SQT1659

Plasmid

#78743

Purpose

Human expression plasmid for AsCpf1-NLS-3xHA

Depositor

Keith Joung

Publication

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mammalian codon-optimized Cpf1-NLS-3xHA

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CAG

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SQT1659

Plasmid

#78743

Purpose

Human expression plasmid for AsCpf1-NLS-3xHA

Depositor

Keith Joung

Publication

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mammalian codon-optimized Cpf1-NLS-3xHA

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CAG

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p5E-kdrl

Plasmid

#78687

Purpose

kdrl promoter for Gateway cloning

Depositor

Jau-Nian Chen, Sarah Childs

Publication

Choi et al Dev Biol. 2007 Apr 15;304(2):735-44. Epu

Insert

kdrl promoter (kdrl Zebrafish)

Use

Unspecified

Promoter

kdrl

Available since

June 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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