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Cytosolic proteostasis through importing of misfolded proteins into mitochondria.
Ruan L, Zhou C, Jin E, Kucharavy A, Zhang Y, Wen Z, Florens L, Li R
Nature. 2017 Mar 16;543(7645):443-446. doi: 10.1038/nature21695. Epub 2017 Mar 1. PubMed Article

Plasmids from Article

ID Plasmid Purpose
91951pFA6a-GFP11-His3MX Universal C-terminal GFP11 tagging
91952p404-Grx5-GFP1-10Expresses GFP1-10 in the mitochondrial matrix
91953GST-HA-GFP11-N1Expresses GST-HA-GFP11 in mammalian cells
91954FlucWT-HA-GFP11-N1Expresses FlucWT-HA-GFP11 in mammalian cells
91955FlucSM-HA-GFP11-N1Expresses aggregation-prone FlucSM-HA-GFP11 in mammalian cells
91956FlucDM-HA-GFP11-N1Expresses aggregation-prone FlucDM-HA-GFP11 in mammalian cells
91957MTS-mCherry-GFP1-10-Hyg-N1Expresses mCherry-GFP1-10 in mammalian cell mitochondria
91958p404-MTS-mCherry-GFP1-10Expresses mCherry-GFP1-10 in yeast cell mitochondria

Antibodies from Article